Background Ferroptosis is a form of programmed cell death characterized by severe dysregulation in the production and degradation of lipid reactive oxygen species (ROS). Peroxynitrite (ONOO−) is one of the most significant reactive oxygen species and a key pathogenic factor in cellular damage. Therefore, monitoring ONOO− levels is of great...

Background Drug-induced liver injury (DILI) is a pathological process in which drugs cause liver dysfunction or damage. Carboxylesterase (CE) is an important enzyme primarily found in the human liver, and its reduced activity or deficiency is associated with the occurrence and severity of DILI. Therefore, detecting changes in CE levels...

Superoxide anion (O2•−), as the primary precursor of reactive oxygen species (ROS), is closely associated with ferroptosis-mediated epilepsy. Therefore, establishing an efficient and convenient method for real-time dynamic monitoring of superoxide anion (O2•−) during ferroptosis in epilepsy is of great importance for the diagnosis and treatment of ferroptosis-mediated epilepsy. However,...

Cytochrome P450 3A4 (CYP3A4) is the most important cytochrome P450 enzyme for drug metabolism in humans. Its activity directly determines the efficacy and safety of over 50% of clinical pharmaceuticals. Traditional detection methods relying on mass spectrometry are limited by high costs and complex workflows, restricting their deep application in complex biological...

To address the challenge that most chiral metabolites lack chromophores and are difficult to detect with high sensitivity, Professor Min Junzhe’s team at Yanbian University has developed a novel fluorescent chiral derivatization reagent—DBD-M-Pro. This reagent integrates both a fluorescent group and a chiral center, enabling precise labeling of amino-containing chiral...

Drug overdose can lead to drug-induced liver injury (DILI), thereby exacerbating liver failure. Extensive research has shown that a rapid increase in peroxynitrite (ONOO⁻) levels is considered a key biomarker of early-stage liver injury. Therefore, real-time monitoring of peroxynitrite dynamics can provide deep insights into its specific roles in processes...