To address the challenge that most chiral metabolites lack chromophores and are difficult to detect with high sensitivity, Professor Min Junzhe’s team at Yanbian University has developed a novel fluorescent chiral derivatization reagent—DBD-M-Pro. This reagent integrates both a fluorescent group and a chiral center, enabling precise labeling of amino-containing chiral compounds and significantly enhancing detection sensitivity. It serves as both a highly selective fluorescent probe and an excellent sensitizing derivatization reagent for mass spectrometry.

Preliminary studies showed that DBD-M-Pro achieves chiral separation of 17 DL-amino acids on a reversed-phase C18 column, with resolution (Rs) values ranging from 1.59 to 24.11, demonstrating outstanding chiral selectivity. The team further applied it to analyze saliva samples from 40 healthy volunteers and found significant differences between males and females in D-Pro, D-Lys, and the D/L-amino acid ratios of five amino acids (p < 0.01–0.001). These findings confirm the reagent’s suitability for highly sensitive trace analysis of amino-functionalized chiral metabolites in biological samples.

Building on this work, the team extended the application of DBD-M-Pro to hair sample analysis, establishing the first UHPLC-HRMS method for simultaneous detection of free chiral amino acids in hair. The method was applied to 30 diabetic patients and 30 healthy controls. Results showed that D-Ala levels were significantly elevated in hair samples from both male and female diabetic patients, and male patients also exhibited significantly higher levels of L-Thr, L-Val, and L-Leu compared to healthy controls (p < 0.05). This study not only confirms that DBD-M-Pro effectively enhances MS detection sensitivity, but also marks the first time that free DL-amino acids in human hair have been proposed as potential non-invasive biomarkers for diabetes.

Product Advantages

• High Purity: 98%;

• High Resolution: DBD-M-Pro achieves complete baseline separation for 17 amino acids and phenylethylamine (PEA) with resolution values ranging from 1.59 to 24.11 (Figure 1);

• Strong Applicability: Can also be used as a mass spectrometry probe, cleaving into specific fragment ions in MS to enhance mass spectrometry response (Figure 2);

• High Sensitivity: Applicable for trace analysis of free DL-amino acids in biological samples (Figure 1, Figure 3);

Professor Min Junzhe, Ph.D. in Pharmaceutical Sciences, Professor and Discipline Leader at Yanbian University, is recognized as a Jilin Province "Top 100 High-level Innovation and Entrepreneurship Talent", "Tumen River Scholar", and "Western Light" Visiting Scholar. He serves as Visiting Professor at the University of Shizuoka, Japan, and Associate Editor of Frontiers in Chemistry. His research focuses on high-sensitivity MS probes for chiral metabolomics and glycomics, mechanistic studies of traditional medicines, and non-invasive diagnostic kits for chronic diseases. He has directed three MEXT Japan projects and three additional Japan-funded programs, led/completed four NSFC projects and four provincial/ministerial projects, authored 130+ SCI papers, and holds six invention patents (three Japan, three China). He has mentored over ten outstanding Ph.D. graduates, including "Young Tumen River Scholar" recipients.

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