Repurposed from a prokaryotic adaptive immune response, the CRISPR/Cas9 system has become a powerful RNA-guided genome-editing tool for targeting and manipulating virtually any genomic sequence, since its inception in 2012.  Nowadays CRISPR is being used in a plethora of applications including the elucidation of gene function involved in disease, the...

The Wurtz coupling uses sodium metal and two alkyl halides to make a new carbon-carbon bond. First, a metal-halogen exchange forms a carbanion that then attacks the second alkyl halide in SN2 fashion. Reagents: Sodium Metal, Solvent (THF, Et2O, Dioxane, Xylenes) Reactant: Alkyl Halide Product: Alkane Type of Reaction: Metal-Catalyzed...

The Yamaguchi esterification converts a carboxylic acid to an ester. First, triethylamine (Et3N) deprotonates the carboxylic acid, which then attacks the Yamaguchi reagent (2,4,6-trichlorobenzoyl chloride) to produce an acid anhydride. The Yamaguchi reagent is released when the acid anhydride is attacked by 4-dimethylaminopyridine (DMAP). The alcohol displaces DMAP and a...

Probe Na-8, 90%Cat. No.: 2698428Appearance: Off-white powder Why is it important to track GSH fluctuation? Chemotherapy is currently the main treatment for cancer, but tumor cells may develop resistance to chemotherapy regimens. Drug resistance is mediated by multiple factors, among which, high levels of glutathione (GSH) is surmised to be a...

The Biginelli reaction is a one-pot (three-component) procedure that in the presence of an acid catalyst, converts a β-keto ester, an aryl aldehyde, and urea to a hexahydropyrimidine derivative. First, urea reacts with the aldehyde affording an aminal, which dehydrates to an N-acyliminium ion. The enol form of the β-keto...

The Dakin-West reaction uses a base to convert an amino acid and anhydride to an acylamino ketone. Acylation of both the nitrogen atom and the carboxylic acid group occur first, followed by an intramolecular acyl substitution, producing a protonated oxazolone intermediate. Further acylation of the oxazolone, acetoxy group additions, and...