The traditional way of connecting nucleic acids and antibodies mainly relies on the modification and reconnection of chemical coupling agents, such as azidating the sugar chain at the Fc end of the antibody, and then performing a coupling reaction with the DBCO-modified nucleic acid at the 5' end to complete the connection between the nucleic acid and the antibody. . This method can control the coupling position, but base modification and other purification steps are costly, and it is difficult to control the number of couplings (Figure 1).

Figure 1 There are many shortcomings in the traditional coupling method of nucleic acids and antibodies.

The AND linker developed by Professor Xing Hang of Hunan University is a bifunctional fusion protein containing two functional domains (Figure 2):

Specific single-stranded DNA (ssDNA) linking domain, which can covalently bind to the sequence 5'-AAGTATTACCAGAAA-3' (underlined is the cleavage binding site) (react with the 5'-phosphate group after cleavage) );
The Fc-terminal connecting domain of the specific antibody can be stably coupled to the Fc-terminal, ultimately forming an antibody-single-stranded DNA conjugate with a stoichiometric ratio of 1:1 (Figure 3).

Figure 2 AND linker contains two functional domains

Figure 3 1:1 quantitative coupling of target antibody and ssDNA

Product advantages

No need to modify the ssDNA chain, stable covalent connection;

The reaction system is stable;

1h efficient reaction;

Specifically linked to the Fc end of the antibody;

Precisely control the stoichiometric ratio of antibody-DNA connection to 1:1.

AND Linker Can be used for immuno-PCR（Immuno-PCR）、Proximity ligation analysis of protein interactions（Proximity Ligation Assay, PLA）Waiting for research.

Bailingwei provides the AND linker developed by Professor Xing Hang.

Professor Xing Hang works at the Institute of Chemical Biology and Nanomedicine, the State Key Laboratory of Chemical Biological Sensing and Metrology and the School of Chemistry and Chemical Engineering of Hunan University. His research focuses on the intersection of inorganic chemistry, nanomedicine and chemical biology. He hopes to develop new chemical methods and material tools based on DNA, polymers and inorganic crystal materials to provide deeper insights into unknown biological processes and precision drug development.

Product name:

AND linker(Antibody-DNA linker), 90%

Quickly connect (single-stranded DNA-antibody Fc end selective quick ligation reagent)

Item number:984538