In addition to the four common bases, DNA also has various chemical modifications, such as cytosine methylation modification, hydroxymethylation modification, aldehyde modification, adenine methylation modification, etc.

Deoxyuracil (dU) is a DNA modified base, which can be formed from cytosine-amidamidine under the action of deaminase or from the misincorporation of dUMP during DNA replication. Misincorporation of dUMP is one type of endogenous DNA damage that can lead to genome instability.

Cytosine deamination at specific regions by deaminases plays a crucial role in cell growth and is closely related to disease. In mammalian cells, uracil can be regulated and removed by various uracil DNA glycosylases, thereby initiating the base excision repair pathway. Therefore, high-resolution mapping of deoxyuracil is of great significance [1] .

J&K Scientific provides dU detection kits developed by Professor Zhou Xiang of Wuhan University.

Product advantages:

• Highly selective markers;
• Reaction conditions are mild.

This kit is mainly used to draw dU genome-wide profiles, and is also used to study the biological functions of dU in the development and progression of diseases such as breast cancer.

Professor Zhou Xiang, currently working at the School of Chemistry and Molecular Sciences of Wuhan University, is the chief scientist of 973; he is responsible for 5 projects and topics. Research work closely revolves around nucleic acids, including deoxyribonucleic acid DNA and ribonucleic acid RNA. The research direction is at the forefront of bioorganic chemistry and chemical biology, with the goal of developing new diagnostic strategies and technical research for major diseases.

References

1. Yafen Wang, Xiong Zhang, Shaoqing Han, Wei Yang, Xiang Zhou. ACS Cent. Sci. 2021, 7, 973−979.