Chemotherapy is currently the main means of treating cancer, but tumor cells may develop drug resistance, and the mechanism is complex. High levels of reduced glutathione (GSH) may be an important reason for this phenomenon. To reveal the detailed role of GSH in chemoresistance, it is crucial to find a reliable live cell detection method.

The scientific research team of Associate Professor Li Xin took advantage of the sensitivity of the electron-deficient C(2p)-S(3p) to the nucleophilic attack of GSH and constructed a small-scale probe library based on click chemical synthesis method, and screened out a most functional probe library. The powerful new fluorescent probe Na-8 can be used to detect endogenous GSH content in living cells.

Figure 1 Detection principle: Na-8 penetrates the cell membrane and is nucleophilically attacked by GSH in the cell to form a linker, thereby remaining in the cell [1][1][1].

Professor Li Xin once worked in the laboratory of Professor E.J. Corey (postdoctoral fellow) and is now engaged in medicinal chemistry research at Zhejiang University. In response to the problem that most biomolecules are difficult to detect in situ in vivo, her laboratory designs fluorescent probes based on the chemical reactivity of biomolecules under the guidance of the drug-like principle, providing sensitive and reliable detection tools for in-depth study of the molecular mechanisms of related pathological processes. and technology.

J&K Scientific exclusively sells the fluorescent probe Na-8 developed by Associate Professor Li Xin:

Product number: 2698428

Na-8, 90%
2 MG 5 MG

References

1. Y. Jiang, J. Cheng. An Ultrasensitive Fluorogenic Probe for Revealing the Role of Glutathione in Chemotherapy Resistance. Chem Sci, 2017, 8(12), 8012-8018.