In the rapidly advancing fields of molecular diagnostics and protein analysis, achieving efficient, precise, and quantitative antibody–DNA conjugation remains a critical challenge affecting assay sensitivity, reproducibility, and overall performance. Conventional chemical conjugation methods are often labor-intensive, expensive, and difficult to control, frequently resulting in heterogeneous conjugates and inconsistent experimental outcomes.
Based on the proprietary AND Linker technology developed by Prof. Hang Xing's research team at Hunan University, we are proud to introduce our upgraded IgG Antibody–DNA Conjugation Kit—a complete, ready-to-use solution designed to simplify antibody–DNA conjugation while delivering exceptional consistency and performance.
From a Core Reagent to a Complete Solution
1. Ready to Use
The kit contains all essential components required for antibody–DNA conjugation, including:
- Lyophilized AND Linker
- Optimized reaction buffer
- Quality control ssDNA
- Nuclease-free water
All reagents have been carefully optimized and pre-configured, eliminating time-consuming protocol optimization and enabling rapid experimental setup.
2. Reliable and Reproducible
An optimized proprietary buffer system ensures stable reaction conditions and delivers highly reproducible conjugation results across experiments.
3. Built-in Quality Control
The included quality control ssDNA allows users to validate conjugation efficiency immediately, providing confidence that the reaction system is functioning correctly before downstream applications.
Powered by the Proven AND Linker Technology
At the heart of the kit is the patented AND Linker, a bifunctional fusion protein originally developed by Prof. Hang Xing's research team.
Precise Fc-Specific Targeting
The AND Linker specifically binds to the Fc region of IgG antibodies without interfering with the antigen-binding Fab domains, preserving the antibody's native biological activity.
Quantitative 1:1 Conjugation
The technology enables precise 1:1 antibody-to-DNA conjugation, producing highly homogeneous conjugates and improving signal consistency for quantitative assays.
Cost-Effective Workflow
Unlike conventional conjugation strategies, the kit requires no chemically modified ssDNA. The AND Linker forms a stable covalent linkage directly with unmodified DNA, eliminating expensive oligonucleotide modification and purification steps while significantly reducing overall experimental costs.
Fast and Efficient
The conjugation reaction can be completed in approximately one hour, greatly improving laboratory efficiency without sacrificing performance.
Applications
Ultra-Sensitive Immuno-PCR
Combine the specificity of antibody-based detection with the extraordinary amplification capability of PCR to detect ultra-low-abundance biomarkers, supporting early disease diagnosis, biomarker discovery, and drug development.
Proximity Ligation Assay (PLA)
Generate high-quality antibody–DNA conjugates for PLA applications, enabling visualization of protein–protein interactions and protein localization at the single-cell level.
Multi-Omics Research
Easily prepare antibody–DNA conjugates for integrated protein and nucleic acid analysis, facilitating next-generation multi-omics workflows.
Development of Novel Diagnostic Probes
Support the development of advanced multiplexed diagnostic platforms, including liquid-phase microarrays, DNA barcoding technologies, and other high-throughput molecular detection systems.
Key Advantages at a Glance
- Complete, ready-to-use kit with all required reagents
- Fc-specific antibody recognition without affecting antigen binding
- Precise 1:1 antibody–DNA conjugation
- No modified ssDNA required
- Stable covalent conjugation
- Excellent reproducibility
- Built-in quality control system
- One-hour workflow
- Reduced experimental cost
- Ideal for Immuno-PCR, PLA, multi-omics, and multiplex diagnostics
The IgG Antibody–DNA Conjugation Kit transforms antibody–DNA conjugation from a complex chemical process into a streamlined, reliable workflow. By combining the innovative AND Linker technology with a fully optimized reagent system, the kit delivers precise, quantitative, and reproducible conjugation while significantly reducing both time and cost, empowering researchers to accelerate discoveries in molecular diagnostics, proteomics, and advanced biomedical research.
艳 李
