Adenosine 5'-triphosphate disodium salt hydrate, 98%, White

Product Introduction

Adenosine 5'-triphosphate disodium salt hydrate (ATP) is a fundamental high-energy nucleotide that serves as the primary energy carrier in living cells. It plays a central role in numerous biological processes, including metabolism, signal transduction, biosynthesis, and cellular regulation.

ATP functions as a universal energy source by releasing energy upon hydrolysis to ADP or AMP, driving a wide range of enzyme-catalyzed reactions. In addition, ATP acts as a substrate for kinases and is involved in cell signaling pathways, particularly through purinergic receptors.

Due to its essential biological role, ATP is widely used in biochemical assays, enzyme activity studies, cell viability testing, and molecular biology research.

Mechanism of Reaction

Adenosine 5'-triphosphate disodium salt hydrate functions as both an energy donor and enzyme substrate.

In biochemical reactions:

• ATP undergoes hydrolysis to ADP + Pi or AMP + PPi
• Releases free energy to drive biochemical processes
• Serves as a substrate for kinase-mediated phosphorylation reactions

In assay systems:

• ATP is utilized by enzymes such as luciferase
• Produces light in the presence of luciferin (bioluminescence)
• Light intensity is proportional to ATP concentration

Key Research Applications

1.ATP-Based Bioluminescence Assays

ATP is widely used in luciferase-based detection systems.Typical applications include:

• Cell viability and cytotoxicity assays
• Microbial contamination detection
• ATP quantification assays

2.Enzyme Activity and Kinase Assays

ATP serves as a universal phosphate donor.Applications include:

• Kinase activity assays
• Phosphorylation studies
• Signal transduction research

3.Cell Biology and Metabolism Studies

ATP is essential for studying cellular energy status.Applications include:

• Cellular metabolism analysis
• Mitochondrial function studies
• Energy balance research

4.Molecular Biology Applications

ATP is widely used in nucleic acid-related enzymatic reactions.Applications include:

• DNA/RNA synthesis
• Ligase reactions
• PCR-related enzymatic systems

Recommended Experimental Conditions

Conditions should be optimized depending on assay system and enzyme requirements.

Advantages in Enzyme Assays

• Universal energy molecule in biological systems
• High sensitivity in ATP-dependent detection assays
• Compatible with a wide range of enzymes
• Suitable for real-time and high-throughput analysis
• Widely validated in research and diagnostics

Storage & Handling

Keep container tightly closed in a dry and well-ventilated place
Keep in a cool place
Recommended storage temperature -20 °C
Heat sensitive

Research Areas

Researchers working in the following fields may benefit from this compound:

• Cell biology and energy metabolism research
• Enzymology and kinase activity studies
• Molecular biology and nucleic acid research
• Clinical and diagnostic biochemistry
• Pharmaceutical and life science research

What is ATP used for in research?
It is used as a universal energy source and enzyme substrate in a wide range of biochemical and cellular assays.

How is ATP detected in assays?
Commonly through luciferase-based bioluminescence, where light intensity correlates with ATP concentration.

Why is ATP important in cell studies?
Because it reflects cellular energy status and viability, making it a key indicator in biological systems.