Trifluoroacetic Acid (TFA, CAS No. 76-05-1) is a strong fluorinated carboxylic acid widely used in peptide synthesis, protein chemistry, pharmaceutical development, organic synthesis, analytical chemistry, and fluorine chemistry. Owing to its high acidity, volatility, and excellent solubility in both water and organic solvents, TFA has become one of the most important reagents in modern synthetic and analytical laboratories. It is extensively employed as a deprotection reagent, ion-pairing reagent for HPLC, reaction solvent, and catalyst in a broad range of chemical transformations.
| Property | Value |
|---|---|
| Chemical Name | Trifluoroacetic Acid |
| Synonyms | TFA; 2,2,2-Trifluoroacetic Acid; Trifluoroethanoic Acid |
| CAS Number | 76-05-1 |
| Molecular Formula | C₂HF₃O₂ (CF₃COOH) |
| Molecular Weight | 114.02 g/mol |
| Appearance | Colorless, transparent liquid |
| Boiling Point | Approximately 72 °C |
| Solubility | Miscible with water and most polar organic solvents |
Trifluoroacetic Acid exhibits several properties that make it indispensable in laboratory research:
Trifluoroacetic Acid (TFA) is a versatile reagent widely used in organic synthesis due to its strong acidity and excellent solvent compatibility. It serves as an effective catalyst and reaction medium for a variety of acid-promoted transformations, helping improve reaction efficiency, selectivity, and product yield.
Typical applications include:
TFA is one of the most widely used reagents in solid-phase peptide synthesis (SPPS). It efficiently removes acid-labile protecting groups and cleaves peptides from solid supports, enabling the synthesis and purification of complex peptide molecules.
Typical applications include:
Trifluoroacetic Acid is commonly used as a mobile-phase modifier, solvent additive, and acidifying reagent in chromatographic analysis. It improves chromatographic peak shape, enhances separation efficiency, and supports reliable analysis of biomolecules and small organic compounds.
Typical analytical techniques include:
TFA is widely employed in pharmaceutical research and process development for the synthesis, purification, and modification of drug candidates and active pharmaceutical ingredients (APIs). It also facilitates the preparation of pharmaceutically relevant intermediates and salts.
Typical applications include:
Due to its strong acidity and chemical stability, Trifluoroacetic Acid is used in environmental and analytical laboratories for sample preparation and chromatographic analysis. It assists in the detection, separation, and quantitative analysis of trace compounds and environmental contaminants.
Typical applications include:
| Parameter | Recommended Conditions |
|---|---|
| Storage | Store in a tightly sealed container |
| Storage Temperature | Room temperature in a cool, dry place |
| Moisture Protection | Recommended |
| Compatible Solvents | Water, methanol, acetonitrile, dichloromethane |
| Handling | Use in a well-ventilated laboratory or fume hood |
Trifluoroacetic Acid is widely used in solid-phase peptide synthesis (SPPS), HPLC mobile phases, organic synthesis, pharmaceutical research, and protein analysis.
Its strong acidity efficiently removes acid-labile protecting groups and cleaves peptides from solid supports while being easily removed because of its volatility.
TFA improves chromatographic peak shape, suppresses unwanted ionic interactions, and enhances the separation of peptides and proteins in reversed-phase HPLC.
Yes. TFA is commonly used during HPLC method development; however, because it may suppress ionization efficiency in electrospray ionization (ESI), lower concentrations or alternative additives such as formic acid may be preferred for LC-MS applications.
Yes. It is widely employed in peptide purification, protein characterization, proteomics sample preparation, and amino acid sequencing workflows.
TFA is commonly used in deprotection, hydrolysis, cyclization, condensation, rearrangement, and other acid-catalyzed organic reactions.
Store it in a tightly sealed container in a cool, dry, well-ventilated location, away from incompatible chemicals and moisture.
Yes. This product is intended for laboratory research, analytical testing, and industrial R&D applications only.
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