Camphorquinone, 98%, Bright yellow solid

Product Introduction

Sodium Pyruvate (CAS No. 10373-78-1) is the sodium salt form of pyruvic acid and an important metabolic intermediate, biochemical reagent, and cell culture supplement widely used in life science research, molecular biology, and biochemistry.

As a key metabolite at the intersection of glycolysis, the tricarboxylic acid (TCA) cycle, and amino acid metabolism, sodium pyruvate serves as a readily available carbon source and energy substrate in biological systems. It is commonly added to cell culture media to support cell growth, maintain metabolic activity, and reduce oxidative stress.

Due to its biological compatibility and antioxidant properties, Sodium Pyruvate is widely applied in cell culture, enzyme assays, metabolic studies, stem cell research, and biochemical analysis.

Mechanism / Principle

Metabolic Role and Antioxidant Function

Sodium Pyruvate participates in cellular metabolism and redox regulation.

Working principle:

1. Sodium pyruvate dissociates into pyruvate ions in aqueous solutions.
2. Pyruvate enters metabolic pathways as an end product of glycolysis.
3. It can be converted into acetyl-CoA and enter the TCA cycle for energy production.
4. Pyruvate can also act as a reducing agent by scavenging reactive oxygen species (ROS).

Key features:

• Supports cellular energy metabolism
• Provides an alternative carbon source
• Helps reduce oxidative stress
• Participates in metabolic pathway regulation

Key Research Applications

1. Cell Culture Supplement

Sodium Pyruvate is widely used as a component of cell culture media.

Applications include:

• Mammalian cell culture
• Stem cell culture
• Primary cell maintenance
• Cell growth optimization
• Serum-free media formulation

2. Metabolic Research

Sodium Pyruvate is an important tool for studying cellular metabolism.

Applications include:

• Glycolysis studies
• TCA cycle research
• Mitochondrial metabolism analysis
• Cellular energy metabolism studies
• Metabolic pathway investigation

3. Biochemical Assays

Used as a substrate or metabolite standard in biochemical experiments.

Applications include:

• Enzyme activity assays
• Metabolic enzyme studies
• Lactate dehydrogenase (LDH) assays
• Pyruvate metabolism research

4. Antioxidant and Oxidative Stress Studies

Sodium Pyruvate is investigated for its ability to regulate oxidative conditions.

Applications include:

• ROS-related studies
• Oxidative stress models
• Cell protection research
• Redox biology experiments

5. Pharmaceutical and Biomedical Research

Sodium Pyruvate supports studies related to cellular function and disease mechanisms.

Applications include:

• Drug screening models
• Disease mechanism research
• Mitochondrial dysfunction studies
• Cell-based assays

Advantages

• Biologically relevant metabolic compound
• High water solubility
• Suitable for cell culture applications
• Supports cellular energy metabolism
• Provides antioxidant activity
• Compatible with biochemical assays

Technical Notes

Note: Concentration requirements vary depending on cell type, culture medium, and experimental objectives.

Storage & Handling

• Store in a cool, dry place.
• Keep container tightly sealed.
• Protect from moisture.
• Store at recommended temperature according to product grade.
• Prepare solutions using sterile techniques for cell culture applications.

Sodium Pyruvate solutions should be prepared under appropriate conditions to maintain biological activity.

Research Areas

Researchers working in the following fields may benefit from Sodium Pyruvate:

• Cell biology
• Molecular biology
• Biochemistry
• Metabolism research
• Stem cell research
• Mitochondrial biology
• Pharmaceutical research

Q1: What is Sodium Pyruvate?

A: Sodium Pyruvate (CAS No. 10373-78-1) is the sodium salt of pyruvic acid and a key metabolic reagent used in biological and biochemical research.

Q2: What is Sodium Pyruvate used for?

A: It is commonly used for:

• Cell culture supplementation
• Metabolic studies
• Enzyme assays
• Oxidative stress research

Q3: Why is Sodium Pyruvate added to cell culture media?

A: Sodium Pyruvate provides an additional energy source and may help protect cells from oxidative stress.

Q4: What role does Sodium Pyruvate play in metabolism?

A: Pyruvate is a central metabolite linking glycolysis with the TCA cycle and mitochondrial energy production.

Q5: Is Sodium Pyruvate an antioxidant?

A: Yes. Pyruvate can react with reactive oxygen species and is commonly studied for its antioxidant effects in biological systems.

Q6: Can Sodium Pyruvate be used in stem cell research?

A: Yes. It is frequently used as a supplement in stem cell and mammalian cell culture systems.

Q7: What is the difference between Pyruvic Acid and Sodium Pyruvate?

Q8: How should Sodium Pyruvate be stored?

A: Store in a sealed container in a dry environment and avoid contamination during handling.