Product Name
Adenosine, 99%, reference material
Molecular Formula
C10H13N5O4
Certificate of Analysis (COA)
IUPAC Name
(2R,3R,4S,5R)-2-(6-aminopurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol
InChI Key
OIRDTQYFTABQOQ-KQYNXXCUSA-N
SMILES
C1=NC(=C2C(=N1)N(C=N2)C@H3C@@H(C@@H(C@H(O3)CO)O)O)N
Product Introduction
Adenosine is a naturally occurring nucleoside composed of the purine base adenine linked to a ribose sugar. It plays a central role in numerous biological processes, including energy transfer, signal transduction, and regulation of cardiovascular and nervous system functions. Due to its versatile biological activity, adenosine is widely used in biochemical research, pharmacology, and molecular biology studies.
Adenosine serves as a key molecule for enzyme assays, receptor studies, metabolic pathway investigations, and drug discovery related to purinergic signaling.
Mechanism of Action
Adenosine can participate in various biochemical and cellular processes:
-
Receptor binding: Acts on adenosine receptors (A1, A2A, A2B, A3) to modulate cellular signaling
-
Metabolic conversion: Can be phosphorylated to AMP, ADP, and ATP, linking it to cellular energy metabolism
-
Enzyme assays: Serves as a substrate or regulator in nucleoside metabolism studies, including adenosine deaminase, adenosine kinase, and other nucleotide-processing enzymes
- Detection is commonly performed via:
-
UV absorbance at 260 nm (typical nucleoside detection)
-
HPLC for quantitative analysis
-
Mass spectrometry for precise identification and quantification
Typical Applications
1.Cardiovascular Research
Adenosine is a clinically used antiarrhythmic agent for the conversion of paroxysmal supraventricular tachycardia. In basic research, adenosine is used to study:
- Atrioventricular node conduction regulation mechanisms
- Coronary blood flow regulation
- Ischemic preconditioning protective effects
- Myocardial energy metabolism
2.Neuroscience
As an important neuromodulator, adenosine is widely used in nervous system research:
- Sleep-wake cycle regulation mechanisms (adenosine is a recognized sleep homeostasis regulator)
- Neuroprotective effects in seizures
- Neuroprotection mechanisms after cerebral ischemia/stroke
- Learning, memory, and synaptic plasticity studies
- Neuroinflammation regulation research
3.Cell Signaling Research
- Adenosine receptor (A₁, A₂A, A₂B, A₃) signaling pathway studies
- cAMP signaling cascade regulation
- Protein kinase activation studies
4.Energy Metabolism Research
Adenosine and its phosphorylated derivatives (AMP, ADP, ATP) are core tools in cellular energy metabolism research:
- Mitochondrial function studies
- Cellular energy status assessment
- Metabolic pathway regulation research
5.Drug Screening & Development
- Adenosine receptor agonist/antagonist screening
- Adenosine kinase inhibitor development
- Adenosine deaminase inhibitor studies
- Cardiovascular drug, neuroprotective agent, and anti-inflammatory drug development
6.Enzymology Studies
Adenosine serves as substrate or product for multiple enzymes:
- Adenosine deaminase (ADA) – converts adenosine to inosine
- Adenosine kinase (ADK) – phosphorylates adenosine to AMP
- S-adenosylhomocysteine hydrolase (SAHH) – participates in methionine cycle
Product Features
- High purity: ≥99.8%, meeting requirements for research and diagnostic reagent development
- Natural endogenous molecule: Good biocompatibility, high physiological relevance
- Multi-disciplinary applications: Covers cardiovascular, neurological, metabolic, and drug development research
- Multiple specifications: Available in various pack sizes for laboratory research to industrial production needs
Advantages in Enzyme Assays
- Direct substrate or regulator for nucleoside-metabolizing enzymes
- No need for fluorescent labeling in standard UV-based assays
- Cost-effective tool for enzyme kinetics and metabolic studies
- Well-established in nucleoside metabolism and receptor research literature
Recommended Experimental Conditions
| Parameter |
Recommended Condition |
| Detection Wavelength |
260 nm (UV), or enzyme-coupled 340 nm |
| Buffer System |
PBS, Tris-HCl, or HEPES, pH 7.0–8.0 |
| Temperature |
25–37°C |
| Assay Format |
UV-Vis, HPLC, LC-MS, microplate enzyme assays, cell-based assays |
Research Areas
Researchers working in the following fields may benefit from this compound:
- Purine metabolism research
- Nucleoside and nucleotide biochemistry
- Cell signaling and receptor studies
- Pharmaceutical and drug discovery research
- Molecular and cellular biology
