Product Name
Gentamicin sulfate, ≥590 IU/mg
Molecular Formula
C21H43N5O7·H2SO4
Certificate of Analysis (COA)
Synonyms
Gentamicin solution
IUPAC Name
(2R,3R,4R,5R)-2-(1S,2S,3R,4S,6R)-4,6-diamino-3-(2R,3R,6S)-3-amino-6-(1-aminoethyl)oxan-2-yloxy-2-hydroxycyclohexyloxy-5-methyl-4-(methylamino)oxane-3,5-diol;(2R,3R,4R,5R)-2-(1S,2S,3R,4S,6R)-4,6-diamino-3-(2R,3R,6S)-3-amino-6-(aminomethyl)oxan-2-yloxy-2-hydroxycyclohexyloxy-5-methyl-4-(methylamino)oxane-3,5-diol;(2R,3R,4R,5R)-2-(1S,2S,3R,4S,6R)-4,6-diamino-3-(2R,3R,6S)-3-amino-6-1-(methylamino)ethyloxan-2-yloxy-2-hydroxycyclohexyloxy-5-methyl-4-(methylamino)oxane-3,5-diol;sulfuric acid
InChI Key
RDEIXVOBVLKYNT-VQBXQJRRSA-N
SMILES
CC(C@@H1CCC@H(C@H(O1)OC@@H2C@H(CC@H(C@@H(C@H2O)OC@@H3C@@H(C@H(C@@(CO3)(C)O)NC)O)N)N)N)N.CC(C@@H1CCC@H(C@H(O1)OC@@H2C@H(CC@H(C@@H(C@H2O)OC@@H3C@@H(C@H(C@@(CO3)(C)O)NC)O)N)N)N)NC.CC@@1(COC@@H(C@@H(C@H1NC)O)OC@H2C@@H(CC@@H(C@H(C@@H2O)OC@@H3C@@H(CCC@H(O3)CN)N)N)N)O.OS(=O)(=O)O
Product Introduction
Gentamicin Sulfate (CAS No. 1405-41-0) is a broad-spectrum aminoglycoside antibiotic widely used in microbiology, molecular biology, and cell culture research.
Gentamicin is a mixture of related aminoglycoside compounds produced by Micromonospora species. It exhibits antibacterial activity by targeting bacterial ribosomes and interfering with protein synthesis.
In laboratory research, Gentamicin Sulfate is commonly used as an antibiotic selection reagent, contamination control agent, and microbiological research tool, particularly in mammalian cell culture, recombinant protein expression systems, and bacterial studies.
Mechanism / Principle
Bacterial Protein Synthesis Inhibition
Gentamicin exerts antibacterial effects by disrupting bacterial translation.
Working principle:
- Gentamicin enters susceptible bacterial cells.
- It binds to the 30S ribosomal subunit.
- Ribosomal function is disrupted, causing incorrect decoding of mRNA.
- Abnormal proteins are produced, leading to bacterial growth inhibition or cell death.
Key features:
- Targets bacterial ribosomes
- Effective against many Gram-negative bacteria
- Used for selective antimicrobial control
- Supports contamination prevention in cell culture
Key Research Applications
1. Cell Culture Antibiotic Selection
Gentamicin Sulfate is widely used in biological laboratories to reduce bacterial contamination.
Applications include:
- Mammalian cell culture
- Primary cell culture
- Recombinant protein expression systems
- Routine contamination prevention
2. Microbiology Research
Gentamicin is an important tool in bacterial studies.
Applications include:
- Antimicrobial susceptibility studies
- Bacterial growth inhibition assays
- Antibiotic resistance research
- Microbial identification experiments
3. Molecular Biology Research
Gentamicin is used in genetic and cellular research workflows.
Applications include:
- Selection of genetically modified cells
- Vector-based research systems
- Recombinant DNA experiments
- Gene expression studies
4. Biotechnology Research
Gentamicin supports controlled biological production systems.
Applications include:
- Recombinant protein production
- Cell line maintenance
- Bioprocess research
- Laboratory biotechnology workflows
5. Antibiotic Resistance Studies
Gentamicin is commonly investigated in antimicrobial research.
Applications include:
- Resistance mechanism studies
- Antibiotic interaction analysis
- Microbial evolution research
- Drug screening studies
Advantages
- Broad antibacterial activity
- Widely used laboratory antibiotic
- Suitable for cell culture applications
- Effective contamination control reagent
- Commonly used in microbiological research
- Compatible with many biological workflows
Storage & Handling
- No special measures required.
Keep container tightly closed in a dry and well-ventilated place
- Containers which are opened must be carefully resealed and kept upright to prevent leakage
- Recommended storage temperature 2 - 8 °C
- Store under inert gas
- Hygroscopic
- Heat sensitive
Research Areas
Researchers working in the following fields may benefit from Gentamicin Sulfate:
- Microbiology
- Molecular biology
- Cell biology
- Biotechnology
- Pharmaceutical research
- Antimicrobial research
- Recombinant protein production
Q1: What is Gentamicin Sulfate?
A: Gentamicin Sulfate (CAS No. 1405-41-0) is an aminoglycoside antibiotic commonly used in microbiology and biological research.
Q2: What is Gentamicin Sulfate used for?
A: It is mainly used for:
- Preventing bacterial contamination in cell culture
- Microbial research
- Antibiotic selection
- Antimicrobial studies
Q3: How does Gentamicin work?
A: Gentamicin binds to the bacterial 30S ribosomal subunit and interferes with protein synthesis, resulting in bacterial growth inhibition.
Q4: Is Gentamicin Sulfate suitable for cell culture?
A: Yes. Gentamicin Sulfate is widely used as an antibiotic supplement in mammalian cell culture systems.
Q5: What bacteria are sensitive to Gentamicin?
A: Gentamicin is particularly active against many Gram-negative bacteria and some Gram-positive bacteria.
Q6: What is the difference between Gentamicin and Gentamicin Sulfate?
A: Gentamicin is the active antibiotic compound, while Gentamicin Sulfate is the sulfate salt form commonly supplied for laboratory and pharmaceutical use.
Q7: How should Gentamicin Sulfate be stored?
A: Store in a sealed container under recommended temperature conditions and protect from moisture.
Q8: Can Gentamicin Sulfate be used for antibiotic resistance studies?
A: Yes. It is frequently used in studies investigating bacterial resistance mechanisms and antimicrobial activity.
National Toxicology Program, Institute of Environmental Health Sciences, National Institutes of Health (NTP). 1992. National Toxicology Program Chemical Repository Database. Research Triangle Park, North Carolina.
