Introduction: The Critical Role of Peroxynitrite (ONOO⁻) in Disease Pathology Peroxynitrite anion (ONOO-) plays a key regulatory role in many physiological processes and is closely related to the occurrence of oxidative damage, ischemic stroke, neurodegenerative diseases, cancer and other diseases.As a highly reactive nitrogen species, ONOO⁻ is implicated in nitrosative...

Changes in intracellular and intracellular pH play an important role in physiological and pathological processes. Changes in intracellular pH can easily induce cell division, rapid growth and functional disorders. The reduction of extracellular pH to acidic is an important signal for cancer and other lesions. Therefore, the detection of pH...

Mitophagy is the process by which cells remove damaged or aged mitochondria and recycle their constituent elements. It is closely related to many physiological and pathological processes such as aging, neurodegenerative diseases, and cancer, so the study of mitophagy is of great significance.Currently, two methods are mainly used to study...

Nitrogen-containing compounds widely exist in natural products, drugs, functional materials and other fields, so the efficient construction of C-N bonds has attracted much attention. Traditionally, the formation of amide groups mainly relies on the homocleavage of N-X bonds, but N-X bonds are difficult to construct under harsh conditions, which fundamentally...

The study of protein phosphorylation (Figure 1) is called phosphoproteomics and is one of the important post-translational modification (PTM) methods in protein research, which mainly occurs on serine, threonine and tyrosine residues. This kind of PTM plays an important role in almost all cell signal transduction pathways, and at least...

According to statistics, more than two-thirds of human proteins can be phosphorylated. The huge signaling network composed of these phosphorylation sites provides important information for people to evaluate the physiological status of cells, identify disease markers, and discover drug targets. Therefore, phosphoproteomics is a very important direction in current protein...