Mitophagy is the process by which cells remove damaged or aged mitochondria and recycle their constituent elements. It is closely related to many physiological and pathological processes such as aging, neurodegenerative diseases, and cancer, so the study of mitophagy is of great significance.Currently, two methods are mainly used to study...
12
Nov
Nitrogen-containing compounds widely exist in natural products, drugs, functional materials and other fields, so the efficient construction of C-N bonds has attracted much attention. Traditionally, the formation of amide groups mainly relies on the homocleavage of N-X bonds, but N-X bonds are difficult to construct under harsh conditions, which fundamentally...
12
Nov
The study of protein phosphorylation (Figure 1) is called phosphoproteomics and is one of the important post-translational modification (PTM) methods in protein research, which mainly occurs on serine, threonine and tyrosine residues. This kind of PTM plays an important role in almost all cell signal transduction pathways, and at least...
12
Nov
According to statistics, more than two-thirds of human proteins can be phosphorylated. The huge signaling network composed of these phosphorylation sites provides important information for people to evaluate the physiological status of cells, identify disease markers, and discover drug targets. Therefore, phosphoproteomics is a very important direction in current protein...
12
Nov
The traditional way of connecting nucleic acids and antibodies mainly relies on the modification and reconnection of chemical coupling agents, such as azidating the sugar chain at the Fc end of the antibody, and then performing a coupling reaction with the DBCO-modified nucleic acid at the 5' end to complete...
24
Sep
More than 170 million people worldwide are infected with hepatitis C virus (HCV), a single-stranded positive-strand RNA virus. Once the human body is infected, it is very likely to develop diseases such as chronic hepatitis, cirrhosis and even liver cancer. Currently, the commonly used methods for detecting HCV infection in...
