Introduction: The Critical Role of Peroxynitrite (ONOO⁻) in Disease Pathology

Peroxynitrite anion (ONOO-) plays a key regulatory role in many physiological processes and is closely related to the occurrence of oxidative damage, ischemic stroke, neurodegenerative diseases, cancer and other diseases.As a highly reactive nitrogen species, ONOO⁻ is implicated in nitrosative stress and cellular damage, making it a critical biomarker for understanding disease mechanisms.
So, how can we find a method for real-time monitoring of endogenous ONOO⁻ levels to explore its biological mechanisms in disease pathogenesis?

The Breakthrough: NP3 Two-Photon Fluorescent Probe

The scientific research team of Associate Professor Li Xin of Zhejiang University has developed a two-photon fluorescent probe for detecting ONOO- content in living cells and vivo - NP3 (λex 375nm; λem 470nm), which leads to a dearomatization reaction by oxidizing p-hydroxyaniline structures. Generates strong fluorescence to detect ONOO- content (Figure 1).
One of the most significant advantages of NP3 is its excellent biocompatibility and ability to cross the blood-brain barrier. This enables real-time monitoring of nitrosative stress during ischemic and hemorrhagic cerebrovascular injury in living mice, providing unprecedented insights into stroke pathology.

Figure 1 NP3 AND ONOO- Reaction schematic

Key Product Advantages of NP3

• High sensitivity: detection limit as low as 5nM;
• Fast response: reaction can reach equilibrium within seconds;
• Strong specificity: other oxidizing species have no obvious response to the probe;
• Within the pH range of 6-8, the fluorescent product is less affected by pH.

Professor Xin Li conducted her postdoctoral research in the laboratory of Nobel Laureate, Professor E.J. Corey. She is currently engaged in medicinal chemistry research at Zhejiang University. Research Focus: Her laboratory focuses on addressing the significant challenge of in-situ detection of biomolecules in vivo. Guided by the drug-like principle, her team designs fluorescent probes based on the specific chemical reactivity of target biomolecules. This innovative approach provides sensitive and reliable tools for elucidating the molecular mechanisms of disease pathology.

Notice for Bulk/Industrial Orders

J&K Scientific is the exclusive distributor for the two-photon fluorescent probe NP3, developed by Professor Xin Li：

• #CAS:1808274-52-3    NP3, 90%

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