The cell membrane is not just the boundary of a cell—it is a dynamic information exchange platform. Changes in fine structures such as pseudopodia, microfilaments, intercellular nanotubes, and migrasomes often reveal key laws governing cellular life activities. Bio-Vision Technology introduces a high-performance fluorescent probe – RMG3, a powerful tool designed for high-resolution, long-term cell membrane imaging.
Core Advantages of RMG3
RMG3 is a fluorescent probe based on a modified rhodamine B fluorophore, specifically designed to target the cell membrane. It offers exceptional fluorescence brightness, a fast and wash-free protocol, broad cellular applicability, sharper imaging, and higher chemical stability.
Comparison with DiO (a traditional green fluorescent cell membrane probe):
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RMG3 enables faster staining with a wash-free protocol
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RMG3 anchors to the cell membrane for extended periods
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RMG3 has significantly slower internalization rates, providing more accurate membrane localization over time
Comparison with Wheat Germ Agglutinin (WGA) conjugates:
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RMG3 targets the phospholipid bilayer, offering better cell-type universality
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As a small-molecule compound, RMG3 has higher chemical stability
Flexible Applications
RMG3 enables clear fluorescence imaging of the cell membrane and its fine structures, including pseudopodia, microfilaments, intercellular nanotubes, and migrasomes. It provides an effective, reliable, and convenient tool for studying cell membrane morphology and dynamics.
Excitation/emission wavelength: 570/595 nm
Compatible channels: FITC, TRITC, Cy3, Texas Red
Key Application Scenarios
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Cell membrane morphology and dynamics studies
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Cell migration, invasion, and pseudopodia formation analysis
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Intercellular communication (e.g., nanotube) research
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Exploration of novel organelles such as migrasomes
Frequently Asked Questions (FAQ)
Q1: What makes RMG3 different from DiO?
A1: Unlike DiO, RMG3 offers a wash-free staining protocol, much slower internalization, and longer membrane anchoring time. This provides more accurate and stable cell membrane imaging, especially for long-term live-cell studies.
Q2: Do I need to wash cells after staining with RMG3?
A2: No. RMG3 is designed for rapid, wash-free staining. Simply add the probe to your culture medium, incubate briefly, and image directly.
Q3: Can RMG3 be used for multicolor imaging?
A3: Yes. RMG3 has excitation/emission at 570/595 nm, making it compatible with green (FITC), orange (TRITC, Cy3), and red (Texas Red) channels for simultaneous imaging of multiple targets.
Q4: Does RMG3 work in fixed cells?
A4: Yes. RMG3 performs well in both live cells and fixed cells, offering flexibility across different experimental models.
Q5: How does RMG3 compare to WGA conjugates?
A5: RMG3 targets the phospholipid bilayer rather than glycoproteins or glycolipids, giving it better universality across different cell types. As a small-molecule probe, RMG3 also has superior chemical stability compared to protein-based WGA conjugates.
Q6: Can RMG3 stain fine membrane structures like pseudopodia and nanotubes?
A6: Absolutely. RMG3's excellent brightness and low background enable high-resolution imaging of delicate structures such as pseudopodia, microfilaments, intercellular nanotubes, and migrasomes.
Q7: Is RMG3 suitable for long-term live-cell imaging?
A7: Yes. RMG3 has significantly slower internalization than DiO, allowing it to anchor to the cell membrane for extended periods. This makes it ideal for long-term tracking of membrane dynamics.
Q8: What types of studies can RMG3 be used for?
A8: RMG3 is widely used in cell membrane morphology studies, cell migration and invasion assays, intercellular communication research, and exploration of novel organelles such as migrasomes.
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艳 李
