A Breakthrough in Peroxynitrite Detection

Peroxynitrite (ONOO⁻) is one of the most oxidizing and cytotoxic reactive nitrogen species, playing a critical role in numerous pathological processes including inflammation, neurodegenerative diseases, and ischemia-reperfusion injury. However, due to its extremely short half-life, site-specific generation (particularly enriched in mitochondria), and coexistence with other reactive oxygen/nitrogen species (ROS/RNS), achieving highly selective, sensitive, and spatially localized detection of ONOO⁻ in complex biological systems has long been a major technical challenge.

Traditional ONOO⁻ probes suffer from poor targeting (unable to focus on mitochondria), shallow penetration (visible light range), and susceptibility to signal interference.

Now, J&K Scientific introduces MITO-CC – a high-fidelity ratiometric fluorescent probe for ONOO⁻, combining mitochondrial targeting with two-photon imaging to deliver high selectivity and high sensitivity. It precisely captures intracellular ONOO⁻, eliminating signal interference and maximizing signal fidelity.

Key Features – Why MITO-CC Is an Excellent Research Tool

High Selectivity – No Signal Interference

MITO-CC exhibits exceptional specificity for ONOO⁻, with no significant cross-reactivity toward various reactive species including H₂O₂, H₂S₂, HOCl, O₂⁻, SO₃²⁻, and others. This resolves the signal interference issues commonly seen with traditional probes.

High Sensitivity – Rapid Capture of Dynamic Changes

MITO-CC reliably responds to low concentrations of ONOO⁻ (stable detection in the 0–25 µM range). The signal response is fast and stable, enabling easy capture of dynamic fluctuations of intracellular ONOO⁻.

Precise Mitochondrial Targeting

MITO-CC precisely targets cellular mitochondria, providing an accurate tool for studying mitochondrial-related oxidative stress and inflammatory mechanisms.

Dual-Mode Imaging: Two-Photon + Ratiometric Imaging

• Two-photon excitation (800 nm) enables deep tissue penetration.

• Ratiometric fluorescence imaging (F₄₇₈/F₆₅₄) provides high-resolution imaging in cells, tissue sections, and live inflammation models.

These scenarios are all within its capabilities!

• ROS/RNS detection

• Inflammation, oxidative stress & mitochondrial function

• Cell/tissue/in vivo imaging with high-specificity ONOO⁻ probe

J&K Scientific provides a stable supply of MITO-CC, the ONOO⁻ tracer developed by Researcher Cheng Dan from Affiliated Nanhua Hospital, University of South China.

Dr. Cheng Dan, Ph.D., Principal Investigator, Doctoral Supervisor Dr. Cheng Dan has long been engaged in research on intelligent molecular probes and their applications in clinical medical imaging. She has led multiple research projects, including those funded by the National Natural Science Foundation of China (NSFC). As first or corresponding author, she has published over 30 SCI papers in prestigious international journals such as the Journal of the American Chemical Society (JACS), Chemical Science, Analytical Chemistry, and Advanced Healthcare Materials, including three highly cited papers. From 2022 to 2025, she has been consecutively named to the World's Top 2% Most Influential Scientists list published by Stanford University.

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