Mass spectrometry instrumentation now finds applications across more than 60% of economic sectors and has been explicitly recognized as an industrial key enabling technology with fundamental and widespread utility. Among its critical supporting reagents, mass spectrometry derivatization reagents represent a breakthrough opportunity for achieving technological leapfrogging in analytical detection.

TMT (Tandem Mass Tag) is a novel class of mass spectrometry derivatization reagents independently developed by the research team of Prof. Fengguo Xu at China Pharmaceutical University. These reagents are designed for targeted derivatization and subsequent MS detection of compounds containing functional groups such as carboxyl, phosphate, amine, phenol hydroxyl, and carbonyl—including pharmaceuticals, small-molecule endogenous metabolites, stimulants, and genotoxic impurities.

By enabling the analysis of 37% of substances that are non-ionizable or poorly ionizable by ESI (electrospray ionization), TMT reagents help push the performance limits of mass spectrometers and can enhance overall detection performance by up to 63%.

Key Advantages of TMT Derivatization Reagents

1. Dramatically Enhanced Sensitivity

Sensitivity improvements of up to 2000-fold have been achieved in mass spectrometry detection.

2. Superior Performance Over Conventional Reagents

TMT reagents deliver over 300 times greater signal enhancement compared to traditional reagents such as:

• Dansyl chloride (Dns‑Cl)

• Dansyl hydrazine (Dns‑Hz)

• Dansyl piperazine (Dns‑PP)

3. Separation of Isomers

When coupled with ion mobility mass spectrometry (IM‑MS), TMT reagents enable effective separation and detection of isomeric compounds.

4. Absolute Quantification Without Reference Standards

Through a “one-side, multi-evaluation” strategy, TMT technology allows for absolute quantification of analytes even in the absence of reference standards.

5. Enhanced Spatial Resolution

TMT-assisted workflows empower mass spectrometry to advance from tissue-level analysis down to cellular and even subcellular resolution.

Fengguo Xu, Ph.D. Supervisor Dean, School of Traditional Chinese Pharmacy, China Pharmaceutical University Deputy Director, Key Laboratory of Drug Quality and Safety Early Warning, Ministry of Education
Academic Honors & Appointments: Distinguished Professor of Jiangsu Province New Century Excellent Talent, Ministry of Education Jiangsu Outstanding Youth Scholar Leading Talent, Jiangsu Innovation & Entrepreneurship Team Recipient, Jiangsu Youth Science & Technology Award
Professional Affiliations: Deputy Director, Chinese Medicine Analysis Branch, Chinese Association of Chinese Medicine Deputy Secretary-General, Metabolomics Branch, Chinese Biophysical Society Deputy Secretary-General, Drug Analysis Technology Branch, Chinese Medical Biotechnology Association

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