5-Carboxyfluorescein is widely utilized in research focused on:
Fluorescent Probes: This compound serves as an effective fluorescent marker in biological assays, allowing researchers to visualize cellular processes and track biomolecules in real-time.
pH Indicators: Due to its pH-sensitive fluorescence, it is used in various applications to measure acidity levels in solutions, making it valuable in environmental monitoring and food safety testing.
Drug Delivery Systems: Its properties enable it to be incorporated into drug formulations, enhancing the tracking of drug release and distribution within biological systems, which is crucial in pharmaceutical research.
Immunoassays: The compound is commonly employed in enzyme-linked immunosorbent assays (ELISAs) for the detection of specific proteins, aiding in clinical diagnostics and research.
Bioconjugation: It is often used to label antibodies and other biomolecules, facilitating studies in proteomics and molecular biology by improving the detection and analysis of target molecules.
