Product Introduction

D-(+)-Trehalose Dihydrate (CAS No. 6138-23-4) is a naturally occurring non-reducing disaccharide composed of two glucose molecules linked by an α,α-1,1-glycosidic bond.

Due to its exceptional ability to stabilize biological structures, proteins, membranes, and cells under stress conditions, Trehalose is widely used as a bioprotective agent, stabilizer, cryoprotectant, and formulation additive in life science, pharmaceutical, biotechnology, cosmetics, and food research.

Unlike reducing sugars, Trehalose does not participate in Maillard reactions, providing excellent chemical stability and making it valuable for protecting sensitive biomolecules during freeze-drying (lyophilization), storage, and transportation.

Structural Characteristics

Non-Reducing Disaccharide Structure

Trehalose consists of two glucose units connected through an α,α-1,1 linkage.

This structure provides:

• High chemical stability
• Resistance to oxidation
• Low reactivity with proteins
• Protection against molecular degradation

Hydrophilic Hydroxyl Groups

Multiple hydroxyl groups enable:

• Strong hydrogen bonding
• Water retention ability
• Biomolecule stabilization
• Membrane protection

Dihydrate Crystal Form

The dihydrate structure provides:

• Stable crystalline properties
• Improved handling performance
• Good storage stability
• Controlled hydration characteristics

Key Research Applications

1. Protein and Biomolecule Stabilization

Trehalose is widely used as a stabilizing excipient for sensitive biological molecules.

Applications include:

• Protein formulation
• Enzyme stabilization
• Antibody preservation
• Vaccine formulation research
• Recombinant protein storage

Trehalose protects proteins by replacing water molecules and maintaining structural integrity during drying or storage.

2. Cryoprotection and Lyophilization

Trehalose is an important cryoprotective agent in biotechnology.

Applications include:

• Freeze-dried biological products
• Cell preservation
• Microorganism preservation
• Tissue engineering research
• Biological sample storage

It reduces damage caused by freezing and dehydration stress.

3. Pharmaceutical Formulations

Trehalose is widely investigated as a pharmaceutical excipient.

Applications include:

• Injectable formulations
• Biologics stabilization
• Drug delivery systems
• Vaccine development
• Lyophilized pharmaceutical products

Its excellent biocompatibility makes it suitable for advanced pharmaceutical formulations.

4. Cell Biology and Biotechnology

Trehalose is used in biological research to protect cells and cellular components.

Applications include:

• Cell preservation
• Organelle stabilization
• Microbial preservation
• Stem cell research
• Biotechnology processes

5. Cosmetics and Personal Care

Trehalose is used as a moisturizing and protective ingredient.

Applications include:

• Skin hydration formulations
• Anti-aging products
• Moisturizing systems
• Protective cosmetic formulations

Its water-binding ability helps maintain skin moisture balance.

6. Food Science and Nutritional Research

Trehalose is widely used in food-related applications.

Applications include:

• Food stabilization
• Texture improvement
• Flavor preservation
• Functional food development
• Sugar replacement research

Advantages

• Excellent biomolecule stabilization ability
• Non-reducing sugar with low chemical reactivity
• High water retention capability
• Strong cryoprotective performance
• Compatible with biological systems
• Suitable for pharmaceutical and biotechnology applications

Storage & Handling

• Store in a cool place.
• Keep container tightly closed in a dry and well-ventilated place.
• Containers which are opened must be carefully resealed and kept upright to prevent leakage.
• Store under inert gas. Moisture sensitive.Hygroscopic

Research Areas

• Biotechnology
• Pharmaceutical development
• Protein science
• Cell preservation
• Vaccine research
• Cosmetics science
• Food chemistry

Q1: What is D-(+)-Trehalose Dihydrate?

A: D-(+)-Trehalose Dihydrate (CAS No. 6138-23-4) is a naturally occurring non-reducing disaccharide used as a stabilizer and protective agent for biological materials.

Q2: What is Trehalose Dihydrate used for?

A: It is mainly used in:

• Protein stabilization
• Vaccine formulation
• Lyophilization
• Cell preservation
• Pharmaceutical excipient research
• Cosmetic formulations

Q3: Why is Trehalose used in protein stabilization?

A: Trehalose forms hydrogen-bond interactions with biomolecules and helps maintain protein structure during dehydration, freezing, and storage.

Q4: What is the difference between Trehalose and sucrose?

Q5: Is Trehalose used in vaccine formulations?

A: Yes. Trehalose is widely studied as a stabilizer for vaccines and biologics because it helps protect proteins during freeze-drying and storage.

Q6: Can Trehalose protect cells during freezing?

A: Yes. Trehalose is commonly used as a cryoprotective compound to reduce damage caused by freezing and dehydration.

Q7: Why is Trehalose suitable for cosmetics?

A: Its strong water-binding ability helps maintain moisture and protect skin cells from environmental stress.

Q8: How should Trehalose Dihydrate be stored?

A: Store in a sealed container under dry conditions and protect from humidity to maintain product quality.