Product Name
β-Nicotinamide adenine dinucleotide disodium salt, 98%, reduced
Molecular Formula
C21H27N7Na2O14P2
Certificate of Analysis (COA)
Synonyms
- Coenzyme I reduced disodium salt
- Diphosphopyridine nucleotide reduced disodium salt
IUPAC Name
disodium;(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-ylmethoxy-oxidophosphoryl (2R,3S,4R,5R)-5-(3-carbamoyl-4H-pyridin-1-yl)-3,4-dihydroxyoxolan-2-ylmethyl phosphate
InChI Key
QRGNQKGQENGQSE-WUEGHLCSSA-L
SMILES
C1C=CN(C=C1C(=O)N)C@H2C@@H(C@@H(C@H(O2)COP(=O)(O-)OP(=O)(O-)OCC@@H3C@H(C@H(C@@H(O3)N4C=NC5=C(N=CN=C54)N)O)O)O)O.Na+.Na+
Product Introduction
β-Nicotinamide Adenine Dinucleotide Disodium Salt (β-NAD⁺ Disodium Salt, CAS No. 606-68-8) is the disodium salt form of nicotinamide adenine dinucleotide (NAD⁺), an essential biological coenzyme involved in cellular oxidation–reduction reactions and energy metabolism.
NAD⁺ functions as a critical electron carrier by accepting and transferring electrons between metabolic pathways. It plays a central role in glycolysis, the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, DNA repair, and cellular signaling processes.
Due to its biological importance, β-NAD⁺ Disodium Salt is widely used in biochemistry, enzymology, molecular biology, metabolism research, pharmaceutical studies, and diagnostic reagent development.
Mechanism / Principle
Redox Cofactor Function
β-NAD⁺ acts as a reversible electron acceptor and donor in enzymatic reactions.
Working principle:
- NAD⁺ accepts electrons and hydrogen ions from substrates.
- It is converted into the reduced form NADH.
- NADH transfers electrons to respiratory chain components or other enzymes.
- NAD⁺ is regenerated to participate in additional metabolic reactions.
Key features:
- Essential biological electron carrier
- Supports oxidation–reduction reactions
- Participates in cellular energy metabolism
- Enables enzyme activity measurement through NAD⁺/NADH conversion
Key Research Applications
1. Enzyme Activity Assays
β-NAD⁺ is widely used as a coenzyme substrate in biochemical assays.
Applications include:
- Dehydrogenase activity assays
- Enzyme kinetics studies
- NAD⁺-dependent enzyme research
- Spectrophotometric detection assays
2. Metabolism Research
NAD⁺ is a central molecule in cellular metabolic pathways.
Applications include:
- Glycolysis studies
- TCA cycle analysis
- Mitochondrial metabolism research
- Cellular energy metabolism studies
3. Molecular Biology and Cell Biology
NAD⁺ participates in important cellular processes.
Applications include:
- DNA repair pathway studies
- Sirtuin enzyme research
- Cellular signaling investigations
- Aging and longevity research
4. Pharmaceutical and Biomedical Research
NAD⁺ metabolism is widely investigated as a therapeutic target.
Applications include:
- Metabolic disease research
- Drug screening assays
- Cellular stress studies
- Disease mechanism investigation
5. Diagnostic Reagent Development
NAD⁺/NADH cycling systems are commonly used in analytical methods.
Applications include:
- Clinical biochemical assays
- Enzyme-linked detection systems
- Metabolite analysis
- Diagnostic reagent formulation
Advantages
- High biological relevance
- Essential cellular coenzyme
- Suitable for enzyme assays
- Supports metabolic pathway research
- Compatible with biochemical detection systems
- Widely used in life science research
Storage & Handling
- Store in a cool place
- Keep container tightly closed in a dry and well-ventilated place
- Containers which are opened must be carefully resealed and kept upright to prevent leakage
- Recommended storage temperature:-20 °C
- Light sensitive. Hygroscopic
Research Areas
Researchers working in the following fields may benefit from β-NAD⁺ Disodium Salt:
- Biochemistry
- Molecular biology
- Cell biology
- Enzymology
- Metabolism research
- Pharmaceutical research
- Clinical biochemistry
Q1: What is β-Nicotinamide Adenine Dinucleotide Disodium Salt?
A: β-NAD⁺ Disodium Salt (CAS No. 606-68-8) is the disodium salt form of NAD⁺, an essential coenzyme involved in cellular redox reactions and energy metabolism.
Q2: What is β-NAD⁺ Disodium Salt used for?
A: It is commonly used for:
- Enzyme activity assays
- Metabolic studies
- Biochemical research
- Diagnostic reagent development
Q3: What is the difference between NAD⁺ and NADH?
A: NAD⁺ is the oxidized form that accepts electrons, while NADH is the reduced form that carries transferred electrons.
Q4: Why is NAD⁺ important in metabolism?
A: NAD⁺ transfers electrons during metabolic reactions and is essential for energy production through glycolysis, the TCA cycle, and oxidative phosphorylation.
Q5: Can β-NAD⁺ Disodium Salt be used in enzyme assays?
A: Yes. NAD⁺ is widely used as a cofactor in dehydrogenase and other NAD⁺-dependent enzyme assays.
Q6: Is β-NAD⁺ Disodium Salt stable in solution?
A: NAD⁺ stability depends on pH, temperature, and storage conditions. Freshly prepared solutions are generally recommended for sensitive assays.
Q7: What is the difference between NAD⁺ and NAD⁺ disodium salt?
A: NAD⁺ disodium salt is the sodium salt form of NAD⁺, offering improved handling and solubility characteristics for laboratory applications.
Q8: How should β-NAD⁺ Disodium Salt be stored?
A: Store sealed at low temperature, protect from light, and avoid repeated freeze–thaw cycles.
