2-Chloro-4-nitrophenyl α-L-fucopyranoside is a chromogenic substrate specifically designed for the detection of α-L-fucosidase activity. Upon enzymatic hydrolysis, it releases 2-chloro-4-nitrophenol, a yellow-colored compound that can be quantitatively measured by spectrophotometry.
This compound is widely used in enzyme activity assays, glycosidase research, and microbiological studies, particularly in systems involving fucose metabolism and α-L-fucosidase-expressing organisms.
Due to its clear colorimetric response and high sensitivity, it is an effective tool for enzyme characterization, metabolic studies, and diagnostic assay development.
2-Chloro-4-nitrophenyl α-L-fucopyranoside functions as a colorimetric enzyme substrate.In enzymatic reactions:
This compound is widely used for quantitative detection of α-L-fucosidase activity.Typical applications include:
It is commonly used in fucose-related metabolic studies.Applications include:
Useful for identifying organisms expressing α-L-fucosidase.Applications include:
Widely used in biochemical and diagnostic applications.Applications include:
| Parameter | Recommended Conditions |
| Detection Method | Colorimetric (spectrophotometric) detection |
| Detection Wavelength | ~405 nm |
| Buffer Systems | Phosphate buffer, citrate buffer |
| Temperature | 25–37 °C |
| Assay Format | Microplate assays, cuvette-based assays |
Optimization may be required depending on enzyme source and assay conditions.
Recommended storage temperature 2 - 8 °C
Researchers working in the following fields may benefit from this compound:
What is this compound used for?
It is used as a chromogenic substrate for detecting α-L-fucosidase activity.
How is enzyme activity measured?
By monitoring the release of 2-chloro-4-nitrophenol, detected at ~405 nm.
Is it suitable for quantitative assays?
Yes, it is widely used in quantitative enzyme assays and high-throughput screening.
