Product Name
Fluorescein isothiocyanate isomer I, 95%
Molecular Formula
C21H11NO5S
Certificate of Analysis (COA)
Synonyms
- FITC
- Fluorescein 5-isothiocyanate
IUPAC Name
3',6'-dihydroxy-6-isothiocyanatospiro2-benzofuran-3,9'-xanthene-1-one
InChI Key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
SMILES
C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
Fluorescein isothiocyanate isomer I is widely utilized in research focused on:
Bioconjugation: This compound is commonly used to label proteins and antibodies for various biological assays, enhancing the visualization of cellular components under fluorescence microscopy.
Immunofluorescence: It plays a crucial role in immunofluorescence techniques, allowing researchers to detect specific antigens in cells or tissue sections, which is vital for diagnostics and research in cell biology.
Flow Cytometry: The chemical is employed in flow cytometry to tag cells, enabling the analysis of cell populations based on their fluorescence properties, which is essential in immunology and cancer research.
Environmental Monitoring: It is used in environmental studies to trace pollutants or toxins in water samples, providing researchers with a powerful tool for assessing ecosystem health.
Drug Development: In pharmaceutical research, it aids in the development of fluorescent probes for drug delivery systems, improving the efficacy of targeted therapies.