Cat. No JK255493_1_G
J&K Chemical

Product Information

Product Name
Fluorescein isothiocyanate isomer I, 95%
Brand Name
J&K
Product Number
255493
CAS
3326-32-7
Molecular Formula
C21H11NO5S
Molecular Weight
389.38
Certificate of Analysis (COA)​
COA not found

General Information

Synonyms
  • FITC
  • Fluorescein 5-isothiocyanate
PubChem CID
18730
IUPAC Name
3',6'-dihydroxy-6-isothiocyanatospiro2-benzofuran-3,9'-xanthene-1-one
InChI Key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
SMILES
C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O

Properties

Melting Point
>360

Safety Information

Signal Word
Danger
Hazard Statement
H317
H334
Precautionary Statement
P261
P272
P280
P321
P501
P284
P302+P352
P333+P317
P362+P364
P304+P340
P342+P316
Storage Condition
Store at 2-8℃

Application

Fluorescein isothiocyanate isomer I is widely utilized in research focused on:

Bioconjugation: This compound is commonly used to label proteins and antibodies for various biological assays, enhancing the visualization of cellular components under fluorescence microscopy.

Immunofluorescence: It plays a crucial role in immunofluorescence techniques, allowing researchers to detect specific antigens in cells or tissue sections, which is vital for diagnostics and research in cell biology.

Flow Cytometry: The chemical is employed in flow cytometry to tag cells, enabling the analysis of cell populations based on their fluorescence properties, which is essential in immunology and cancer research.

Environmental Monitoring: It is used in environmental studies to trace pollutants or toxins in water samples, providing researchers with a powerful tool for assessing ecosystem health.

Drug Development: In pharmaceutical research, it aids in the development of fluorescent probes for drug delivery systems, improving the efficacy of targeted therapies.

References Data Source From Pubchem

Application of extracellular vesicle-carried antimicrobial peptide LL37 enhances rice resistance to Magnaporthe oryzae

Publication Name: Phytopathology Research
Publication Date: 2026-06-23
DOI: 10.1186/s42483-026-00421-0

Correction: Electroacupuncture, Combined with Decellularized Nerve Scaffolds, Enhances Spinal Cord Neuron Protection and Functional Recovery in Sciatic Nerve-Injured Rats via the PI3K/Akt/NGF Pathway

Publication Name: Innovations in Acupuncture and Medicine
Publication Date: 2026-05-29
DOI: 10.1186/s44424-025-00036-w|10.1186/s44424-026-00053-3

Correction: The ANGPTL3-integrin α5 axis drives retinal vascular leakage in diabetic retinopathy

Publication Name: Journal of Translational Medicine
Publication Date: 2026-05-09
DOI: 10.1186/s12967-026-07710-4|10.1186/s12967-026-08229-4

Author Correction: A clinic-responder-derived defined microbial consortium enhances anti-PD-1 immunotherapy efficacy in mice

Publication Name: Nature Microbiology
Publication Date: 2026-03-09
DOI: 10.1038/s41564-026-02279-6|10.1038/s41564-026-02417-0

Oriented nanobody–field-effect transistor interfaces enable ultrasensitive cancer biomarker detection

Publication Name: Nature Sensors
Publication Date: 2026-03-06
DOI: 10.1038/s44460-026-00040-4

Fluorescein isothiocyanate isomer I, 95%

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