D-Glucose 6-phosphate sodium salt, 98%

Product Introduction

D-Glucose 6-phosphate sodium salt (G6P) is a key phosphorylated glucose intermediate involved in central metabolic pathways, including glycolysis, gluconeogenesis, and the pentose phosphate pathway. It is formed by phosphorylation of glucose via hexokinase or glucokinase and serves as a critical branch point in cellular metabolism.

Due to its central biological role, G6P is widely used in enzyme assays, metabolic pathway studies, and biochemical research, particularly in systems involving glucose metabolism and NADP⁺/NADPH-dependent reactions.

In enzymatic assays, G6P is commonly utilized as a substrate for glucose-6-phosphate dehydrogenase (G6PD), where it is oxidized to 6-phosphogluconolactone, generating NADPH, which can be quantitatively measured at 340 nm.

Mechanism of Reaction

D-Glucose 6-phosphate sodium salt functions as a metabolic intermediate and enzyme substrate.In biochemical reactions:

• It serves as a substrate for glucose-6-phosphate dehydrogenase (G6PD)
• G6P is oxidized to 6-phosphogluconolactone
• NADP⁺ is reduced to NADPH
• NADPH exhibits strong absorbance at 340 nm, enabling spectrophotometric detection

Additionally:

• G6P participates in glycolysis (conversion to fructose-6-phosphate)
• It enters the pentose phosphate pathway, supporting biosynthesis and redox balance

The formation of NADPH is directly proportional to enzyme activity.

Key Research Applications

1.Glucose-6-Phosphate Dehydrogenase (G6PD) Assays

G6P is widely used for quantitative determination of G6PD activity in biochemical and clinical research.Typical applications include:

• G6PD enzyme activity assays
• NADPH generation assays
• Redox enzyme activity monitoring

2.Metabolic Pathway Studies

The compound plays a central role in cellular metabolism research.Applications include:

• Glycolysis pathway studies
• Pentose phosphate pathway analysis
• Cellular metabolic flux research

3.Enzyme Kinetics Studies

G6P is frequently used in enzyme kinetics experiments involving metabolic enzymes.Typical uses include:

• Determination of kinetic parameters (Km and Vmax)
• Enzyme activity comparison
• Substrate utilization studies

4.Clinical and Diagnostic Research

Because G6P is involved in key metabolic enzymes, it is widely used in clinical biochemistry and diagnostic reagent development.Applications include:

• G6PD deficiency studies
• Clinical enzyme assays
• Diagnostic kit development

• Recommended Experimental Conditions

  Parameter	Recommended Conditions
  Detection Method	NADPH-based spectrophotometric detection
  Detection Wavelength	340 nm
  Buffer Systems	Tris-HCl buffer, phosphate buffer, or HEPES buffer
  Temperature	25–37 °C
  Assay Format	Spectrophotometric assays, microplate assays, enzyme-coupled assays

• Optimization may be required depending on enzyme source and assay design.

Advantages in Enzyme Assays

• Essential substrate in glucose metabolism assays
• Compatible with NADPH-based detection systems
• Suitable for continuous kinetic monitoring
• Widely used in biochemical and clinical research

Storage & Handling

Store in a cool place. Keep container tightly closed in a dry and well ventilated place.
Containers which are opened must be carefully resealed and kept upright to prevent leakage

Research Areas

Researchers working in the following fields may benefit from this compound:

• Metabolic biochemistry research
• Enzymology and enzyme kinetics
• Cellular energy metabolism studies
• Clinical and diagnostic biochemistry
• Pharmaceutical and life science research

What is D-Glucose 6-phosphate used for?
It is commonly used as a substrate in G6PD enzyme assays and metabolic pathway studies.

How is G6P detected in enzyme assays?
Through NADPH formation, which is measured at 340 nm.

Why is G6P important in metabolism research?
Because it is a central intermediate linking multiple metabolic pathways, including glycolysis and the pentose phosphate pathway.