N-Benzoyl-L-tyrosine ethyl ester, 99%

Product Introduction

N-Benzoyl-L-tyrosine ethyl ester (BTEE) is a widely used chromogenic substrate for the detection of serine protease activity, particularly chymotrypsin. It is an esterified derivative of L-tyrosine, designed to mimic natural peptide substrates recognized by proteolytic enzymes.

Upon enzymatic hydrolysis, BTEE undergoes cleavage of the ester bond, resulting in a measurable change in absorbance, allowing quantitative monitoring of enzyme activity.

Due to its specificity and reliability, BTEE is commonly used in enzyme kinetics studies, protease activity assays, inhibitor screening, and biochemical research, especially in systems involving chymotrypsin-like enzymes.

Mechanism of Reaction

N-Benzoyl-L-tyrosine ethyl ester functions as a substrate for serine proteases.In enzymatic reactions:

• It is hydrolyzed by chymotrypsin and related proteases
• The ester bond is cleaved
• Produces N-benzoyl-L-tyrosine and ethanol

This makes BTEE a classical substrate for studying protease catalysis and enzyme specificity.

Key Research Applications

1.Protease Activity Assays

BTEE is widely used for quantitative measurement of serine protease activity.Typical applications include:

• Chymotrypsin activity assays
• Proteolytic enzyme characterization
• Enzyme activity monitoring

2.Enzyme Kinetics Studies

The compound is ideal for enzyme kinetics analysis.Applications include:

• Determination of kinetic parameters (Km and Vmax)
• Substrate specificity studies
• Reaction mechanism investigation

3.Enzyme Inhibitor Screening

BTEE is commonly used in screening protease inhibitors.Applications include:

• Drug discovery research
• Protease inhibitor evaluation
• Screening of bioactive compounds

4.Biochemical and Pharmaceutical Research

Widely applied in biochemistry and life science research.Applications include:

• Protease-related pathway studies
• Protein digestion research
• Enzyme-based assay development

Recommended Experimental Conditions

Experimental parameters may require optimization depending on enzyme source.

Advantages in Enzyme Assays

• High specificity for chymotrypsin-like proteases
• Enables continuous kinetic monitoring
• Simple and reproducible assay system
• Suitable for inhibitor screening
• Widely validated in enzymology research

Storage & Handling

Keep container tightly closed in a dry and well-ventilated place
Keep in a cool place

Research Areas

Researchers working in the following fields may benefit from this compound:

• Enzymology and protease activity studies
• Biochemistry and protein research
• Pharmaceutical and drug discovery research
• Enzyme kinetics and inhibition studies
• Life science research involving proteolytic enzymes

What is BTEE used for?
It is used as a substrate for measuring chymotrypsin and related protease activity.

How is enzyme activity detected?
By monitoring the increase in absorbance at ~256 nm during substrate hydrolysis.

Is BTEE suitable for inhibitor studies?
Yes, it is widely used in protease inhibitor screening and enzyme kinetics analysis.