Hanks' Buffer is the most commonly used balanced salt solution in biological experiments. It is mainly used for rinsing tissue blocks and cells when collecting samples for cell culture, as well as preparing other reagents. This product is ready-to-use and can be directly used for routine purposes such as washing cells during cell culture after filtration and sterilization. No dilution or filtration sterilization is required before use.
Composition
| Composition | Concentration (mM) |
| NaCl | 137.93 |
| KCl | 5.36 |
| NaHCO3 | 4.17 |
| KH2PO4 | 0.44 |
| Na2HPO4 | 0.34 |
| D-Glucose | 5.56 |
Features
1. Balanced salt solutions commonly used in biological laboratories.
2. It is one of the commonly used buffers in cell isolation or culture.
3. It has the function of maintaining osmotic pressure, keeping PH stable and providing simple nutrition.
4. After 0.1μm sterilizing filtration and endotoxin detection, it can be directly used for routine cell culture. Endotoxin ensure less than 0.5 EU / mL .
5. Without Ca2+&Mg2+.
Product Nature
| PH | 7.4±0.1 |
| Phenol red indicator | No phenol red |
| Sterile | Sterile |
| Osmotic pressure | 270 - 315 mOsm/kg |
| Endotoxin | <0.5EU/mL |
| Cat # | Product Name | Formula | Package |
| HANKS-1000-1 | Hanks,Balanced Salt Solution(1x） | 137.93 mM NaCl, 5.36 mM KCl, 4.17 mM NaHCO₃, 0.441 mM KH2PO4, 0.338 mM Na2HPO4, 5.56 mM D-Glucose [-][-][-][-]Magnesium Chloride | 1000mL/ Bottle, 6 Bottles/Case |
