L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt is a specialized chromogenic substrate designed for the detection of γ-glutamyltransferase (GGT) activity. It contains a glutamic acid moiety linked to a nitroanilide chromophore, enabling colorimetric detection upon enzymatic cleavage.
In enzymatic reactions, this substrate is hydrolyzed by GGT to release a nitroaniline derivative, which produces a measurable color change. This allows for quantitative spectrophotometric monitoring of enzyme activity.
Due to its specificity and sensitivity, this compound is widely used in clinical biochemistry, enzyme assays, diagnostic reagent development, and liver function studies.
L-Glutamic acid γ-(3-carboxy-4-nitroanilide) ammonium salt functions as a chromogenic substrate for GGT.In enzymatic reactions:
This compound is widely used for quantitative determination of GGT activity.Typical applications include:
GGT is an important biomarker in clinical biochemistry.Applications include:
Liver disease research
The substrate is suitable for enzyme kinetics analysis.Applications include:
Widely used in drug development and toxicology.Applications include:
| Parameter | Recommended Conditions |
| Detection Method | Colorimetric (spectrophotometric) assay |
| Detection Wavelength | ~405 nm |
| Buffer Systems | Tris-HCl buffer, phosphate buffer |
| Temperature | 25–37 °C |
| Assay Format | Microplate assays, clinical chemistry analyzers |
Optimization may be required depending on sample type and assay system.
Keep container tightly closed in a dry and well-ventilated place
Keep in a cool place
Recommended storage temperature 2 - 8 °C
Keep in a dry place.
Researchers working in the following fields may benefit from this compound:
What is this compound used for?
It is used as a chromogenic substrate for detecting γ-glutamyltransferase (GGT) activity.
How is enzyme activity measured?
By detecting the release of a nitroaniline chromophore at ~405 nm.
Is it suitable for clinical applications?
Yes, it is widely used in clinical chemistry and diagnostic assay development, especially for liver function analysis.
