Fluorescent reporter groups are the foundation of fluorescent probe development. Their performance directly determines the detection sensitivity and practicality of the probe. Bio-Vision Technology, in collaboration with Professor Qin Wang, introduces an important fluorescein derivative—3-Methoxyfluorescein – leveraging its unique optical properties to empower high-sensitivity detection research.
Outstanding Performance
Ultra-Low Background, High Fluorescence Contrast
Fluorescent probes constructed with 3-Methoxyfluorescein exhibit extremely low intrinsic fluorescence quantum yield, generating virtually no background fluorescence. This minimizes signal interference at the source.
High Fluorescence After Reaction, Maximized Sensitivity
Upon specific reaction with target molecules, the released 3-Methoxyfluorescein displays a high fluorescence quantum yield, producing a significant fluorescence enhancement signal. This perfectly addresses the challenge of insufficient sensitivity in detecting low-concentration target molecules.
Validated by Peer-Reviewed Research
3-Methoxyfluorescein has been successfully used to construct a reaction-type fluorescent probe for hydrogen sulfide (H₂S). The probe is formed by linking the reporter group with a reactive disulfide unit via an ester bond. Upon specific reaction with H₂S, highly fluorescent 3-Methoxyfluorescein is released, generating a significant fluorescence enhancement signal that enables highly selective and sensitive detection of H₂S. This research was published in the top-tier journal Angewandte Chemie International Edition.
A Reliable Choice for Fluorescent Probe Development
From fundamental fluorescent probe development to high-sensitivity detection of various biomolecules and reactive species, 3-Methoxyfluorescein offers the core advantages of low background and high fluorescence contrast. It provides a stable, high-quality reporter group for fluorescent probe construction, helping researchers develop more efficient detection tools.
Frequently Asked Questions (FAQ)
Q1: What is 3-Methoxyfluorescein and how does it work?
A1: 3-Methoxyfluorescein is a fluorescein derivative with extremely low intrinsic fluorescence. It serves as a caged reporter group. When linked to a recognition unit via a cleavable bond (e.g., ester or disulfide), the probe remains non-fluorescent. Upon specific reaction with a target molecule (such as H₂S), the bond is cleaved, releasing free 3-Methoxyfluorescein, which exhibits strong green fluorescence. This "turn-on" mechanism enables high-sensitivity detection with minimal background.
Q2: What are the key advantages of using 3-Methoxyfluorescein over traditional fluorescein?
A2: Traditional fluorescein derivatives often suffer from high intrinsic background fluorescence, which limits detection sensitivity. 3-Methoxyfluorescein offers two major advantages:
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Ultra-low background: The caged form has very low fluorescence quantum yield, reducing false signals.
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High fluorescence enhancement: Upon activation, it produces a strong "turn-on" signal, enabling detection of low-concentration targets with excellent signal-to-noise ratio.
Q3: Has 3-Methoxyfluorescein been validated in real applications?
A3: Yes. It has been successfully used in a reaction-type fluorescent probe for hydrogen sulfide (H₂S) detection. The probe showed high selectivity and sensitivity, with results published in Angewandte Chemie International Edition, a top-tier chemistry journal. This demonstrates its reliability and practical utility for detecting reactive species and biomolecules.
Q4: What types of targets can be detected using 3-Methoxyfluorescein-based probes?
A4: While H₂S detection is a proven example, the design strategy can be extended to other targets. By attaching 3-Methoxyfluorescein to a recognition unit via a cleavable linker specific to a target molecule (e.g., enzyme, reactive sulfur species, metal ion, or pH change), researchers can develop "turn-on" probes for a wide range of analytes.
Q5: Is 3-Methoxyfluorescein suitable for live-cell imaging?
A5: Yes. The low background and high fluorescence enhancement make it well-suited for live-cell imaging applications. The "turn-on" mechanism minimizes non-specific signals, allowing clear visualization of target molecules in biological environments. However, cell permeability and probe delivery should be optimized based on the specific probe design.
Q6: What form does 3-Methoxyfluorescein come in?
A6: Please contact us for current product specifications, including purity, quantity, and available derivatives. We can also provide custom synthesis for specialized probe development projects.
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Professor Qin Wang is affiliated with the College of Chemistry and Environmental Science at Shaanxi University of Technology. |
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| Her research focuses on the construction and application of nano fluorescent probes. She has published multiple research papers in SCI-indexed journals, including nine as the first author. Additionally, she has participated in the completion of two National Natural Science Foundation projects. |
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艳 李
