Key Advantages of DQF Fluorescent Dyes
Compared to traditional rhodamine dyes, DQF fluorescent dyes—including DQF-560, DQF-565, and DQF-584 Figure 1—offer:
Larger Stokes shift → Reduces fluorescence self-quenching, improving imaging clarity.
Better photostability → Ensures long-term fluorescence retention under imaging conditions.
Higher signal-to-noise ratio → Ideal for high-precision imaging.
Enhanced suitability for long-term bioimaging → Applicable to cells, tissues, and in vivo imaging Figures 2 & 3.
These features make DQF fluorescent dyes a powerful tool for biomedical imaging, particularly for applications requiring high contrast and long-term stability.

Figure 1 DQF dyes1
Applications of DQF Fluorescent Dyes
- High-contrast imaging for biological research
- Long-term imaging of living cells and tissues
- In vivo fluorescence imaging with minimal signal loss
J&K Scientific offers high-purity DQF fluorescent dyes, ensuring reliable performance for advanced imaging applications.
Figure 2 (a) Confocal fluorescence images of live HepG2 cells cultured with DQF-570 5.0 μM and Rh6G 5.0 μM with continuous irradiation using confocal microscope with the same parameters. (b) Quantification of the relative mean fluorescence levels of cells from the images of DQF-570 and Rh6G. 1
Figure 3 Intracellular multi-color image of Rh6G 2 μM and LysoDQF-570 2 μM in HepG2 cells simulated with chloroquine 100 μM at different time points. 1
Product list
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Product name:DQF-560, 95% |
Product name:DQF-565, 95% |
Product name:DQF-584, 95% |
Cat. No:9332535 |
Cat. No:9332536 |
Cat. No:9332537 |
Fluorescence properties:λex 560 nm; λem 640 nm |
Fluorescence properties:λex 565 nm; λem 648 nm |
Fluorescence properties:λex 584 nm; λem 660 nm |