Z-Gly-Pro-p-nitroanilide (Z-Gly-Pro-pNA) is a commonly used chromogenic substrate for dipeptidyl peptidase IV (DPP-IV, also known as CD26) and related proline-specific peptidases. The compound consists of a carbobenzoxy (Z) protected glycine–proline dipeptide linked to a p-nitroanilide chromogenic group, enabling convenient colorimetric detection of enzymatic activity.
During enzymatic hydrolysis, DPP-IV cleaves the peptide bond, releasing p-nitroaniline (pNA). The liberated p-nitroaniline produces a yellow chromogenic product, which can be quantitatively measured by spectrophotometry, typically at 405 nm.
Due to its specificity and ease of detection, Z-Gly-Pro-p-nitroanilide is widely used in DPP-IV activity assays, enzyme kinetics studies, inhibitor screening, and biochemical research involving proline-specific peptidases.
Z-Gly-Pro-p-nitroanilide functions as a chromogenic substrate for DPP-IV and related proline-specific peptidases.During the enzymatic reaction:
The increase in absorbance is directly proportional to enzyme activity in the assay system.
Z-Gly-Pro-pNA is widely used for quantitative measurement of DPP-IV activity in biochemical assays.Typical applications include:
The compound is frequently used in enzyme kinetics experiments investigating catalytic properties of proline-specific peptidases.Applications include:
Z-Gly-Pro-pNA is commonly used in screening assays for inhibitors of DPP-IV and related enzymes.Typical uses include:
Because DPP-IV is an important target in metabolic disease research, this substrate is widely used in related biochemical studies.Applications include:
| Parameter | Recommended Conditions |
| Detection Method | Colorimetric spectrophotometric detection |
| Detection Wavelength | 405 nm |
| Buffer Systems | Tris-HCl buffer or phosphate buffer |
| Temperature | 25–37 °C |
| Assay Format | Spectrophotometric assays, microplate reader assays |
Optimization may be required depending on enzyme source and assay design.
Keep container tightly closed in a dry and well-ventilated place
Keep in a cool place
Recommended storage temperature -20 °C
Researchers working in the following fields may benefit from this compound:
What is Z-Gly-Pro-p-nitroanilide used for?
It is commonly used as a chromogenic substrate for dipeptidyl peptidase IV (DPP-IV) activity assays.
What wavelength is used to detect the reaction product?
The released p-nitroaniline is typically measured at 405 nm.
Why is this compound widely used in DPP-IV assays?
Because it provides convenient colorimetric detection of enzyme activity with reliable and reproducible results.
