Enzyme Substrate

Enzyme Substrates in IVD: The Essential Signal Generators

In In Vitro Diagnostic (IVD) systems, Enzyme Substrates are indispensable core components. They act as specialized “signal switches” that convert invisible biochemical reactions into measurable signals.

In enzymatic assays (e.g., ELISA, clinical chemistry reagents), a specific enzyme (such as HRP or ALP) is linked to the detection antibody or analyte. When the colorless substrate (e.g., TMB for HRP, pNPP for ALP) is added, the enzyme catalyzes its conversion into a colored, fluorescent, or luminescent product. The intensity of this signal is directly proportional to the concentration of the target analyte, enabling precise qualitative and quantitative detection.

Applications

  1. Enzyme-Linked Immunosorbent Assay (ELISA)
    Detection of antigens or antibodies.

  2. Clinical Chemistry (Biochemical) Analysis
    Quantitative detection of specific metabolites or enzyme activities in serum/plasma/urine.

  3. Chemiluminescence Immunoassay (CLIA)

  4. Point-of-Care Testing (POCT)

  5. Molecular Diagnostics
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Enzyme

4,6-Ethylidene-4-nitrophenyl-α-D-maltoheptaoside, 98%

General Details CAS # 96597-16-9 SMILES Chemical Purity Molecular Formula C50H77NO38 Chemical Formula Formula Weight 1300.13 Reaction Note Physical Properties Purity 98% Density Melting Point Boiling Point Flash Point Vapor...
$222.88
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α-Ketoglutaric acid, 99%|239270|328-50-7
Enzyme

α-Ketoglutaric acid, 99%

Product Information Product Name α-Ketoglutaric acid, 99% Brand Name J&K Product Number 239270 CAS 328-50-7 Molecular Formula C5H6O5 Molecular Weight 146.10 SDS Document View/Download Certificate of Analysis (COA)​ Search COA...
$19.80
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6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-β-D-glucopyranoside, 98%|1413695|149263-94-5
Enzyme

6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-β-D-glucopyranoside, 98%

Product Information Product Name 6-Methyl-2-pyridinyl 2-(acetylamino)-2-deoxy-1-thio-β-D-glucopyranoside, 98% Brand Name J&K Product Number 1413695 CAS 149263-94-5 Molecular Formula C14H20N2O5S Molecular Weight 328.38 SDS Document View/Download Certificate of Analysis (COA)​ Search COA...
$28.00
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D-Glucose 6-phosphate sodium salt, 98%|178184|54010-71-8
Enzyme

D-Glucose 6-phosphate sodium salt, 98%

Product Information Product Name D-Glucose 6-phosphate sodium salt, 98% Brand Name J&K Product Number 178184 CAS 54010-71-8 Molecular Formula C6H12NaO9P Molecular Weight 282.12 SDS Document View/Download Certificate of Analysis (COA)​...
$16.10
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2-Nitrophenyl β-D-galactopyranoside, 98%|446877|369-07-3
Enzyme

2-Nitrophenyl β-D-galactopyranoside, 98%

Product Information Product Name 2-Nitrophenyl β-D-galactopyranoside, 98% Brand Name J&K Product Number 446877 CAS 369-07-3 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID...
$27.40
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Flavin adenine dinucleotide disodium salt hydrate, 96%|957454|84366-81-4
Enzyme

Flavin adenine dinucleotide disodium salt hydrate, 96%

Product Information Product Name Flavin adenine dinucleotide disodium salt hydrate, 96% Brand Name J&K Product Number 957454 CAS 84366-81-4 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found...
$135.00
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