Enzyme Substrate

Enzyme Substrates in IVD: The Essential Signal Generators

In In Vitro Diagnostic (IVD) systems, Enzyme Substrates are indispensable core components. They act as specialized “signal switches” that convert invisible biochemical reactions into measurable signals.

In enzymatic assays (e.g., ELISA, clinical chemistry reagents), a specific enzyme (such as HRP or ALP) is linked to the detection antibody or analyte. When the colorless substrate (e.g., TMB for HRP, pNPP for ALP) is added, the enzyme catalyzes its conversion into a colored, fluorescent, or luminescent product. The intensity of this signal is directly proportional to the concentration of the target analyte, enabling precise qualitative and quantitative detection.

Applications

  1. Enzyme-Linked Immunosorbent Assay (ELISA)
    Detection of antigens or antibodies.

  2. Clinical Chemistry (Biochemical) Analysis
    Quantitative detection of specific metabolites or enzyme activities in serum/plasma/urine.

  3. Chemiluminescence Immunoassay (CLIA)

  4. Point-of-Care Testing (POCT)

  5. Molecular Diagnostics
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P1,P5-Di(adenosine-5')pentaphosphate trilithium salt|00111|75522-97-3
Enzyme

P1,P5-Di(adenosine-5')pentaphosphate trilithium salt

Product Information Product Name P1,P5-Di(adenosine-5')pentaphosphate trilithium salt Brand Name Chem Impex Product Number 00111 CAS 75522-97-3 Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID 56777378 IUPAC...
$155.30
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Inosine 5'-monophospate disodium salt, 98%|581375|4691-65-0
Enzyme

Inosine 5'-monophospate disodium salt, 98%

Product Information Product Name Inosine 5'-monophospate disodium salt, 98% Brand Name J&K Product Number 581375 CAS 4691-65-0 Molecular Formula C10H11N4Na2O8P Molecular Weight 392.17(anhydrous) SDS Document View/Download Certificate of Analysis (COA)​...
$32.70
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3,3'-Diaminobenzidine, 99%|446581|91-95-2
Enzyme

3,3'-Diaminobenzidine, 99%

Product Information Product Name 3,3'-Diaminobenzidine, 99% Brand Name J&K Product Number 446581 CAS 91-95-2 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID 7071...
$46.30
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Na-Benzoyl-L-arginine ethyl ester hydrochloride|03031|2645-08-1
Arginines, Enzyme

Na-Benzoyl-L-arginine ethyl ester hydrochloride

Product Information Product Name Na-Benzoyl-L-arginine ethyl ester hydrochloride Brand Name Chem Impex Product Number 03031 CAS 2645-08-1 Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID 2723604...
$21.30
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Na-Benzoyl-DL-arginine-4-nitroanilide hydrochloride|01715|911-77-3
Arginines, Enzyme

Na-Benzoyl-DL-arginine-4-nitroanilide hydrochloride

Product Information Product Name Na-Benzoyl-DL-arginine-4-nitroanilide hydrochloride Brand Name Chem Impex Product Number 01715 CAS 911-77-3 Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID 2724371 IUPAC Name...
$34.50
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Z-Gly-Pro-p-nitroanilide, 98%|426683|65022-15-3
Enzyme

Z-Gly-Pro-p-nitroanilide, 98%

Product Information Product Name Z-Gly-Pro-p-nitroanilide, 98% Brand Name J&K Product Number 426683 CAS 65022-15-3 Molecular Formula C21H22N4O6 Molecular Weight 426.40 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$196.50
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