Enzyme Substrate

Enzyme Substrates in IVD: The Essential Signal Generators

In In Vitro Diagnostic (IVD) systems, Enzyme Substrates are indispensable core components. They act as specialized “signal switches” that convert invisible biochemical reactions into measurable signals.

In enzymatic assays (e.g., ELISA, clinical chemistry reagents), a specific enzyme (such as HRP or ALP) is linked to the detection antibody or analyte. When the colorless substrate (e.g., TMB for HRP, pNPP for ALP) is added, the enzyme catalyzes its conversion into a colored, fluorescent, or luminescent product. The intensity of this signal is directly proportional to the concentration of the target analyte, enabling precise qualitative and quantitative detection.

Applications

  1. Enzyme-Linked Immunosorbent Assay (ELISA)
    Detection of antigens or antibodies.

  2. Clinical Chemistry (Biochemical) Analysis
    Quantitative detection of specific metabolites or enzyme activities in serum/plasma/urine.

  3. Chemiluminescence Immunoassay (CLIA)

  4. Point-of-Care Testing (POCT)

  5. Molecular Diagnostics
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Oxamic acid, 98%|121867|471-47-6
Enzyme

Oxamic acid, 98%

Product Information Product Name Oxamic acid, 98% Brand Name J&K Product Number 121867 CAS 471-47-6 Molecular Formula C2H3NO3 Molecular Weight 89.05 SDS Document View/Download Certificate of Analysis (COA)​ Search COA...
$18.70
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5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%|141887|102185-33-1
Enzyme

5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98%

Product Information Product Name 5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98% Brand Name J&K Product Number 141887 CAS 102185-33-1 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found General Information...
$37.80
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Adenosine, 99%|132283|58-61-7
Enzyme

Adenosine, 99%

Product Information Product Name Adenosine, 99% Brand Name J&K Product Number 132283 CAS 58-61-7 Molecular Formula C10H13N5O4 Molecular Weight 267.24 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$25.60
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N-Acetyl-L-cysteine, 99%|114448|616-91-1
Cysteines, Enzyme

N-Acetyl-L-cysteine, 99%

Product Information Product Name N-Acetyl-L-cysteine, 99% Brand Name J&K Product Number 114448 CAS 616-91-1 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID 12035...
$34.40
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Adenosine, 99%, substrate of adenosine deaminase|934909|58-61-7
Enzyme

Adenosine, 99%, substrate of adenosine deaminase

Product Information Product Name Adenosine, 99%, substrate of adenosine deaminase Brand Name J&K Product Number 934909 CAS 58-61-7 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found General...
$25.60
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L-(-)-Fucose, 99%|256603|2438-80-4
Enzyme

L-(-)-Fucose, 99%

Product Information Product Name L-(-)-Fucose, 99% Brand Name J&K Product Number 256603 CAS 2438-80-4 Molecular Formula C6H12O5 Molecular Weight 164.16 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$27.30
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