Product Name
Western Transfer Buffer(10×),PH8.3,500mL/ Bottle, 10 Bottles/Case
Certificate of Analysis (COA)
Western transfer buffers are used in Western blotting (WB) experiments to transfer proteins from gels to membranes, and then to semi-quantitative detection of proteins in complex samples based on antigen-antibody specific binding.
This product is a 10× concentrate and needs to be diluted as follows before use: Take 100 mL of 10× transfer buffer, add 200 mL of anhydrous methanol and mix well, then add distilled water or deionized water to make up to 1 L, mix well and it can be used.
Features
High quality: The buffers are filtered by 0.22 µm to ensure the highest quality.
Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation time while reducing the likelihood of buffer concentration errors.
DNase & RNase free, Protease-free, Pyrogen-free.
Composition
| Tris
| 250 mM
|
| Glycine
| 1.92 M
|
Product Properties
| Appearance
| Colorless clear liquid
|
| pH
| 8.3±0.1 (diluted to 1×)
|
| Sterile
| Non-sterile
|
Storage & Shelf Life
Store at room temperature, valid for two years.
Ordering Information
| Cat # | Description | Package |
| WTB-510-1 | Western Transfer Buffer (10×)
| 500mL/ Bottle |
| WTB-510 | Western Transfer Buffer (10×) | 500mL/ Bottle, 10 Bottles/Case |