Oxamic acid sodium salt is a widely used competitive inhibitor of lactate dehydrogenase (LDH) and a structural analog of pyruvate. Due to its ability to interfere with pyruvate–lactate interconversion, it plays an important role in metabolic enzyme studies and glycolysis-related research.
The compound is commonly applied in enzyme inhibition assays, metabolic pathway analysis, cancer metabolism studies, and biochemical research involving NADH-dependent enzymes.
Because LDH catalyzes the conversion of pyruvate to lactate with concomitant oxidation of NADH to NAD⁺, oxamic acid sodium salt is frequently used to modulate or inhibit this reaction, enabling detailed investigation of enzyme kinetics and metabolic regulation.
Oxamic acid sodium salt functions as a competitive inhibitor of lactate dehydrogenase，During enzymatic systems:
This inhibition allows precise control and analysis of LDH-dependent metabolic pathways.
Oxamic acid sodium salt is widely used for studying LDH inhibition and enzymatic regulation.Typical applications include:
The compound is commonly used in studies of glycolysis and cellular metabolism.Applications include:
Oxamic acid sodium salt is frequently used in enzyme kinetics experiments.Typical uses include:
Because LDH plays a key role in tumor metabolism and hypoxic conditions, this compound is widely used in related studies.Applications include:
| Parameter | Recommended Conditions |
| Detection Method | NADH-based spectrophotometric detection |
| Detection Wavelength | 340 nm |
| Buffer Systems | Tris-HCl buffer, phosphate buffer, or HEPES buffer |
| Temperature | 25–37 °C |
| Assay Format | Spectrophotometric assays, enzyme inhibition assays, microplate assays |
Optimization may be required depending on enzyme source and assay design.
Store in a cool place. Keep container tightly closed in a dry and wellventilated place.
Containers which are opened must be carefully resealed
and kept upright to prevent leakage.
Researchers working in the following fields may benefit from this compound:
What is oxamic acid sodium salt used for?
It is commonly used as a competitive inhibitor of lactate dehydrogenase (LDH) in enzyme and metabolism studies.
How is LDH inhibition monitored?
How is LDH inhibition monitored?
Why is oxamic acid important in metabolism research?
Because it allows selective inhibition of LDH, helping to study glycolysis and metabolic regulation.
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