Sample vial, 2mL, 9 mm, clear glass screw thread vials with write-on spot

Description

• USP type 1 borosilicate glass with solvent-resistant write-on spot for labelling, graduated markings every 0.5mL
• Wide mouth for easy sample filling; increased target area to prevent sampling needle damage
• Standard HPLC/GC vial, compatible with 9mm screw caps
• Compatible with standard autosamplers and robotic arm samplers including Agilent, Shimadzu, Varian

1. How do I choose the right sample vial for my analysis?

Selecting the correct vial depends on four key factors: sample characteristics, analytical method, instrument compatibility, and workflow requirements.

Golden Rule: Always follow the consumable recommendations provided by your instrument manufacturer.

2. What are the differences between crimp, screw, and snap top vials?

3. What is the correct fill volume for a sample vial?

Do not overfill or underfill. Proper fill volume is critical for autosampler performance.

• 2 mL vial → Fill with 1 mL of sample
• 100 µL vial → Fill with 50 µL of sample
• General guideline → Fill to 60–80% of vial capacity

Why headspace matters: The air space in the vial is necessary to avoid forming a vacuum when sample is withdrawn, which could affect reproducibility. Do not inject air into vials to prevent vacuum – this often damages the cap seal.

4. Why does my autosampler report a "missing vial" error?

This error (Error ID: 25471 on Agilent systems) occurs when no vial is found in the position defined in your method.

Probable causes and solutions:

To avoid this error: When loading trays, ensure the tray sits flat and slides backward until it clicks into place. For trays, "A" represents the row and "1" represents the vial position.

5. Can I reuse disposable sample vials?

Generally, no. Disposable vials should be treated as single-use unless explicitly labeled otherwise.

Why not? Pre-rinsed sample reservoirs can interfere with new tests due to leftover contaminants. Even with cleaning, residues may persist and affect trace analysis results.

Exception: If reuse is absolutely necessary and thoroughly validated, clean with appropriate acid washes and certified rinses. However, for chromatography applications, always use new, certified vials.

6. How do I prevent sample contamination from vial handling?

Common mistake: Touching the rim or inner surface of the vial with bare hands.

Prevention tips:

• Always wear powder-free, chemical-resistant gloves
• Handle vials by their bodies only; use clean forceps when possible
• Use certified clean vials for trace/ultra-trace analysis

7. Why is my recovery low or my results inconsistent?

Potential causes and solutions:

8. What is the difference between clear and amber vials?

9. What septa material should I use?

Important: Use of non-pre-slit cap mats and vial caps can cause clogging in the wash drainage lines.

10. How do I store vials properly?

• Store in a clean, dust-free environment using vial racks to prevent breakage
• Protect light-sensitive samples by using amber vials
• Ensure caps are securely tightened to prevent evaporation and leakage
• Do not store vials with loose caps or in positions where they can topple over

11. Should I use certified vials?

Yes for regulated or trace analysis. Certified vials offer:

• ISO "Class 8" cleanroom packing – minimizes particulate contamination
• USP Type I borosilicate glass – low leachability and chemical purity
• PTFE-silicone septa caps – airtight sealing for long-term sample stability
• Regulatory compliance – ISO 15378 certification for pharmaceutical testing

For routine analysis, certified clean, ready-to-use products are sufficient. For trace/ultra-trace analysis, select vials that have undergone stringent cleaning (e.g., tested by Karl Fischer method).

12. Can I use plastic vials instead of glass?

Recommendation: Use glass for chromatography and organic solvents. Plastic may be acceptable for aqueous solutions, storage, and transport. PMP (Polymethylpentene) offers greater inertness and clarity close to glass.