Enzyme Substrate

Enzyme Substrates in IVD: The Essential Signal Generators

In In Vitro Diagnostic (IVD) systems, Enzyme Substrates are indispensable core components. They act as specialized “signal switches” that convert invisible biochemical reactions into measurable signals.

In enzymatic assays (e.g., ELISA, clinical chemistry reagents), a specific enzyme (such as HRP or ALP) is linked to the detection antibody or analyte. When the colorless substrate (e.g., TMB for HRP, pNPP for ALP) is added, the enzyme catalyzes its conversion into a colored, fluorescent, or luminescent product. The intensity of this signal is directly proportional to the concentration of the target analyte, enabling precise qualitative and quantitative detection.

Applications

  1. Enzyme-Linked Immunosorbent Assay (ELISA)
    Detection of antigens or antibodies.

  2. Clinical Chemistry (Biochemical) Analysis
    Quantitative detection of specific metabolites or enzyme activities in serum/plasma/urine.

  3. Chemiluminescence Immunoassay (CLIA)

  4. Point-of-Care Testing (POCT)

  5. Molecular Diagnostics
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2-Chloro-4-nitrophenyl-β-D-maltotrioside, 97%|143875|165522-16-7
Enzyme

2-Chloro-4-nitrophenyl-β-D-maltotrioside, 97%

Product Information Product Name 2-Chloro-4-nitrophenyl-β-D-maltotrioside, 97% Brand Name J&K Product Number 143875 CAS 165522-16-7 Molecular Formula C24H34ClNO18 Molecular Weight 659.98 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$228.00
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4-Nitrophenyl-N-acetyl-β-D-glucosaminide, 98%|549897|3459-18-5
Enzyme

4-Nitrophenyl-N-acetyl-β-D-glucosaminide, 98%

Product Information Product Name 4-Nitrophenyl-N-acetyl-β-D-glucosaminide, 98% Brand Name J&K Product Number 549897 CAS 3459-18-5 Molecular Formula C14H18N2O8 Molecular Weight 342.30 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$30.60
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4,6-Ethylidene-4-nitrophenyl-α-D-maltoheptaoside, 90%|1810804|96597-16-9
Enzyme

4,6-Ethylidene-4-nitrophenyl-α-D-maltoheptaoside, 90%

Product Information Product Name 4,6-Ethylidene-4-nitrophenyl-α-D-maltoheptaoside, 90% Brand Name J&K Product Number 1810804 CAS 96597-16-9 Molecular Formula C50H77NO38 Molecular Weight 1300.1 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$54.80
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3,3',5,5'-Tetramethylbenzidine dihydrochloride|30781|64285-73-0
Enzyme

3,3',5,5'-Tetramethylbenzidine dihydrochloride

Product Information Product Name 3,3',5,5'-Tetramethylbenzidine dihydrochloride Brand Name Chem Impex Product Number 30781 CAS 64285-73-0 Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID 174033 IUPAC Name...
$52.10
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Flavin adenine dinucleotide disodium salt hydrate|00151|84366-81-4
Enzyme

Flavin adenine dinucleotide disodium salt hydrate

Product Information Product Name Flavin adenine dinucleotide disodium salt hydrate Brand Name Chem Impex Product Number 00151 CAS 84366-81-4 Certificate of Analysis (COA)​ Search COA not found General Information PubChem...
$51.50
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Fmoc-4-(Boc-amino)-L-phenylalanine|03755|174132-31-1
Alanines, Enzyme

Fmoc-4-(Boc-amino)-L-phenylalanine

Product Information Product Name Fmoc-4-(Boc-amino)-L-phenylalanine Brand Name Chem Impex Product Number 03755 CAS 174132-31-1 Certificate of Analysis (COA)​ Search COA not found General Information PubChem CID 2756117 IUPAC Name (2S)-2-(9H-fluoren-9-ylmethoxycarbonylamino)-3-4-(2-methylpropan-2-yl)oxycarbonylaminophenylpropanoic...
$107.10
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