Enzyme Substrate

Enzyme Substrates in IVD: The Essential Signal Generators

In In Vitro Diagnostic (IVD) systems, Enzyme Substrates are indispensable core components. They act as specialized “signal switches” that convert invisible biochemical reactions into measurable signals.

In enzymatic assays (e.g., ELISA, clinical chemistry reagents), a specific enzyme (such as HRP or ALP) is linked to the detection antibody or analyte. When the colorless substrate (e.g., TMB for HRP, pNPP for ALP) is added, the enzyme catalyzes its conversion into a colored, fluorescent, or luminescent product. The intensity of this signal is directly proportional to the concentration of the target analyte, enabling precise qualitative and quantitative detection.

Applications

  1. Enzyme-Linked Immunosorbent Assay (ELISA)
    Detection of antigens or antibodies.

  2. Clinical Chemistry (Biochemical) Analysis
    Quantitative detection of specific metabolites or enzyme activities in serum/plasma/urine.

  3. Chemiluminescence Immunoassay (CLIA)

  4. Point-of-Care Testing (POCT)

  5. Molecular Diagnostics
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Phospho(enol)pyruvic acid cyclohexylammonium salt, 98%|211054|10526-80-4
Enzyme

Phospho(enol)pyruvic acid cyclohexylammonium salt, 98%

Product Information Product Name Phospho(enol)pyruvic acid cyclohexylammonium salt, 98% Brand Name J&K Product Number 211054 CAS 10526-80-4 Molecular Formula C9H18NO6P Molecular Weight 267.22 SDS Document View/Download Certificate of Analysis (COA)​...
$14.20
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Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, 98%|925462|911-77-3
Arginines, Enzyme

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, 98%

Product Information Product Name Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, 98% Brand Name J&K Product Number 925462 CAS 911-77-3 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found General Information PubChem...
$99.00
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N-Tosyl-L-alanyloxyindole, 97%|227676|75062-54-3
Enzyme, Indoles

N-Tosyl-L-alanyloxyindole, 97%

Product Information Product Name N-Tosyl-L-alanyloxyindole, 97% Brand Name J&K Product Number 227676 CAS 75062-54-3 Molecular Formula C18H18N2O4S Molecular Weight 358.42 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$33.00
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p-Hydroxybenzoic acid sodium salt, 98%|426669|114-63-6
Benzoic Acids, Enzyme

p-Hydroxybenzoic acid sodium salt, 98%

Product Information Product Name p-Hydroxybenzoic acid sodium salt, 98% Brand Name J&K Product Number 426669 CAS 114-63-6 Molecular Formula C7H5NaO3 Molecular Weight 160.10 SDS Document View/Download Certificate of Analysis (COA)​...
$28.20
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3,3',5,5'-Tetramethylbenzidine, 99%|270119|54827-17-7
Enzyme

3,3',5,5'-Tetramethylbenzidine, 99%

Product Information Product Name 3,3',5,5'-Tetramethylbenzidine, 99% Brand Name J&K Product Number 270119 CAS 54827-17-7 Molecular Formula C16H20N2 Molecular Weight 240.35 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not...
$24.80
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3,3',5,5'-Tetramethylbenzidine dihydrochloride, 98%|292086|64285-73-0
Enzyme

3,3',5,5'-Tetramethylbenzidine dihydrochloride, 98%

Product Information Product Name 3,3',5,5'-Tetramethylbenzidine dihydrochloride, 98% Brand Name J&K Product Number 292086 CAS 64285-73-0 Molecular Formula C16H20N2·2HCl Molecular Weight 313.27 SDS Document View/Download Certificate of Analysis (COA)​ Search COA...
$48.00
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