Enzyme Substrate

Core Substrates and Enzymes — The "Engine" of the Detection Reaction

In In Vitro Diagnostic (IVD) systems, Enzyme Substrates are indispensable core components. They act as specialized “signal switches” that convert invisible biochemical reactions into measurable signals.

In enzymatic assays (e.g., ELISA, clinical chemistry reagents), a specific enzyme (such as HRP or ALP) is linked to the detection antibody or analyte. When the colorless substrate (e.g., TMB for HRP, pNPP for ALP) is added, the enzyme catalyzes its conversion into a colored, fluorescent, or luminescent product. The intensity of this signal is directly proportional to the concentration of the target analyte, enabling precise qualitative and quantitative detection.

Applications

  1. Enzyme-Linked Immunosorbent Assay (ELISA)
    Detection of antigens or antibodies.

  2. Clinical Chemistry (Biochemical) Analysis
    Quantitative detection of specific metabolites or enzyme activities in serum/plasma/urine.

  3. Chemiluminescence Immunoassay (CLIA)

  4. Point-of-Care Testing (POCT)

  5. Molecular Diagnostics

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Phospho(enol)pyruvic acid cyclohexylammonium salt, 98%|211054|10526-80-4
all, Enzyme Substrates

Phospho(enol)pyruvic acid cyclohexylammonium salt, 98%

Product Information Product Name Phospho(enol)pyruvic acid cyclohexylammonium salt, 98% Brand Name J&K Product Number 211054 CAS 10526-80-4 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found Application Product...
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Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, 98%|925462|911-77-3
all, Arginines

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, 98%

Product Information Product Name Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, 98% Brand Name J&K Product Number 925462 CAS 911-77-3 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found Application Product Introduction...
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N-Tosyl-L-alanyloxyindole, 97%|227676|75062-54-3
all, Enzyme Substrates

N-Tosyl-L-alanyloxyindole, 97%

Product Information Product Name N-Tosyl-L-alanyloxyindole, 97% Brand Name J&K Product Number 227676 CAS 75062-54-3 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found Application Product Introduction N-Tosyl-L-alanyloxyindole is...
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p-Hydroxybenzoic acid sodium salt, 98%|426669|114-63-6
all, Benzoic Acids

p-Hydroxybenzoic acid sodium salt, 98%

Product Information Product Name p-Hydroxybenzoic acid sodium salt, 98% Brand Name J&K Product Number 426669 CAS 114-63-6 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found Application Product...
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3,3',5,5'-Tetramethylbenzidine, 99%|270119|54827-17-7
all, Enzyme Substrates

3,3',5,5'-Tetramethylbenzidine, 99%

Product Information Product Name 3,3',5,5'-Tetramethylbenzidine, 99% Brand Name J&K Product Number 270119 CAS 54827-17-7 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found Application Product Introduction 3,3',5,5'-Tetramethylbenzidine (TMB)...
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3,3',5,5'-Tetramethylbenzidine dihydrochloride, 98%|292086|64285-73-0
all, Enzyme Substrates

3,3',5,5'-Tetramethylbenzidine dihydrochloride, 98%

Product Information Product Name 3,3',5,5'-Tetramethylbenzidine dihydrochloride, 98% Brand Name J&K Product Number 292086 CAS 64285-73-0 SDS Document View/Download Certificate of Analysis (COA)​ Search COA not found Application Product Introduction 3,3',5,5'-Tetramethylbenzidine...
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