5-methylcytosine modification (5mC) is one of the important epigenetic modifications in eukaryotic cell genomes. 5mC can be gradually oxidized under the action of TET family enzymes to generate 5-hydroxymethylcytosine (5hmC), 5-aldehydecytosine (5fC) and 5-carboxycytosine (5caC); for the above-mentioned several new DNA modifications Functional research is currently a hot spot in nucleic acid epigenetics.

Among them, the research on 5-aldehyde cytosine (5fC) faces greater challenges. Since the content of 5fC in cell tissues is very small (0.02% - 0.002% cytosine level), scientists first need to find a high-efficiency, high-selectivity method. method to enrich 5fC. All current enrichment methods are based on bisulfite treatment, which is not only costly, but also causes DNA degradation and reduces experimental efficiency [1] .

J&K provides a new 5fC detection kit developed by researcher Yichengqi. It is mainly used for single-base resolution determination of 5fC on the genome; it can also be used to study the biological functions of 5fC in biological development and disease occurrence and development [2] .

Product advantages

• No bisulfite treatment is required, the reaction is mild and will not cause DNA degradation;
• DNA samples containing 5fC sequences are first enriched and then sequenced, greatly reducing sequencing costs;
• It can be applied to the sequencing of precious clinical DNA samples.

Figure 1 Without bisulfite treatment, DNA samples containing 5fC sequences can be enriched before sequencing [1]

 Reference

1. Bo Xia, Dali Han, Xingyu Lu, Zhaozhu Sun, Ankun Zhou, Qiangzong Yin, Hu Zeng, Menghao Liu, Xiang Jiang, Wei Xie, Chuan He, Chengqi Yi. Nat Methods. 2015, 12 (11), 1047-50.
2. Chenxu Zhu, Yun Gao, Hongshan Guo, ..., Kehkooi Kee, Fuchou Tang, Chengqi Yi. Cell Stem Cell. 2017, 20 (5), 720-731.