1. Before use, calibration is necessary, typically every three months. For infrequent use, calibration may be done every six months. Calibration can be achieved through weight calibration, where 1 mL of distilled water at 20°C equals 0.9982 g (using a milligram electronic analytical balance).

  2. Select a pipettor with an appropriate volume range, and it is recommended to operate within 35%-100% of the pipettor's capacity.

  3. Always use pipette tips that are compatible with the pipettor.

  4. Do not rotate the button beyond the maximum or minimum range, as it can jam the mechanical components, causing damage to the pipettor.

  5. Avoid tilting the pipettor.

Some may believe that tilting helps in aspirating liquids more cleanly and provides flexibility. This is absolutely incorrect. The pipetting angle significantly affects accuracy, and deviation from vertical during liquid aspiration or dispensing should not exceed 20°, as it may impact accuracy.

  1. Never use the pipettor to blow or mix liquids.

  2. When aspirating liquid, release the thumb slowly and steadily. Never release suddenly, as rapid aspiration can generate air bubbles, causing liquid to penetrate the internal components of the pipettor, leading to corrosion.

  3. Used pipette tips should not be mixed with new ones to avoid cross-contamination.

  4. Regularly clean the outer surface of the pipettor using 95% alcohol or 60% isopropanol, followed by wiping with distilled water. Allow it to air-dry naturally.

  5. If disinfection is required, the pipettor can be disinfected using UV light or wiped with 75% alcohol. Avoid proximity to open flames.

  6. Store the pipettor in a vertical position. Do not lay it flat. During pipetting operations, liquid may enter the internal parts of the pipettor. When laid flat, these liquids can accumulate on the piston and other components, leading to contamination or corrosion over time.

By 向阳 翟


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