Alkaline Phosphatase (ALP) is associated with human diseases such as those related to bone and liver, making it one of the commonly used labeling enzymes in in vitro diagnostic (IVD) reagents. The main methods for ALP detection are Colorimetry and Continuous Monitoring.
In colorimetric detection, pNPP (4-Nitrophenyl phosphate disodium salt hexahydrate) is a commonly used substrate for ALP. When ALP reacts with pNPP, a yellow water-soluble product is formed, which exhibits light absorption at λ = 405 nm. This reaction is highly sensitive and produces a distinct color, making it widely applicable in the development and production of IVD kits.
BCIP + NBT is one of the ideal chromogenic substrate combinations for ALP. BCIP (5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt) generates a highly reactive product under the catalysis of ALP. This product further reacts with NBT (Nitro Blue Tetrazolium chloride) to form an insoluble deep blue to bluish-purple NBT-formazan, thereby achieving effective labeling.
艳 李
