SDS-polyacrylamide gel electrophoresis (SDS-PAGE) is a method for qualitative protein analysis. It is widely used in molecular biology and protein research to separate oligonucleotides and proteins. J&K Scientific provides SDS-PAGE related reagents, pipettes, and consumables. Experimental Steps Download PDF Experimental Steps I. Preparation of SDS-PAGE Gel Wash the electrophoresis glass...

In the 1980s, with the emergence of electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI)—two soft ionization techniques suitable for protein research—mass spectrometry became a highly important tool for the identification of biological macromolecules. Currently, mass spectrometry is widely used in various fields, including the identification of molecular markers,...

Western blot analysis is a widely used biochemical technique. Its core principle involves transferring protein bands separated by SDS-PAGE onto a solid support membrane for subsequent detection. The procedure generally consists of three main steps: gel electrophoresis, protein transfer (blotting), and immunodetection. Western blot technology continues to evolve, with significant...

Overview of the E. coli Expression System The E. coli expression system is one of the earliest and most established platforms in recombinant protein production. It remains widely used in molecular biology and biotechnology due to its well-characterized genetic background, rapid growth rate, and ability to achieve high-level protein expression....

Bacterial plasmids are commonly used gene delivery vehicles in recombinant DNA technology. Alkaline lysis is a widely employed method for small-scale plasmid DNA preparation. Experimental Steps Download PDF Filter the bacterial culture, transfer the filtrate to a 1.5 mL centrifuge tube, centrifuge at 6000 r/min for 2 minutes, and discard...