Background
Ferroptosis is a form of programmed cell death characterized by severe dysregulation in the production and degradation of lipid reactive oxygen species (ROS). Peroxynitrite (ONOO−) is one of the most significant reactive oxygen species and a key pathogenic factor in cellular damage. Therefore, monitoring ONOO− levels is of great significance in studying diseases such as acute kidney injury mediated by ferroptosis.
Development of NFP-ONOO Probe
Professor Tang Zhixin from Shandong University of Traditional Chinese Medicine has developed a novel near-infrared fluorescent probe, NFP-ONOO, specifically designed for detecting peroxynitrite (ONOO−). It has been successfully applied in cells, zebrafish, and mouse models, demonstrating excellent tissue penetration and biocompatibility, making it suitable for various in vitro and in vivo experiments. NFP-ONOO also enables real-time monitoring of dynamic changes in peroxynitrite during ferroptosis, providing valuable insights into the mechanisms of ferroptosis in diseases such as acute kidney injury. [1]
Product Advantages
• High Sensitivity: Detection limit as low as 10 μM (Figure 1);
• Excellent Tissue Penetration: Fluorescence imaging of endogenous ONOO− in HK-2 cells (Figure 2);
• Biocompatibility: Fluorescence imaging of NFP-ONOO in zebrafish (Figure 3);
• High Purity: ≥90%;
| Tang Zhixin is a Lecturer and Master's Supervisor at Shandong University of Traditional Chinese Medicine, holding a Ph.D. from Dalian University of Technology (2020). His research focuses on developing fluorescent probes for disease biomarkers and their applications in bioimaging, as well as utilizing fluorescence-based methods for screening active components in traditional Chinese medicine. He has led research projects funded by the Shandong Natural Science Foundation and the China Postdoctoral Science Foundation. His achievements include receiving the 2024 Science and Technology Award from the China National Association of Ethnic Medicine and a Silver Prize in the National Postdoctoral Innovation Competition. He has published 13 papers as first or corresponding author in journals such as Biosensors and Bioelectronics, Sensors and Actuators B: Chemical, and Analytical Chemistry. | 
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Related Product:
- #Cat:9423828 NFP-ONOO, 90%
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