{"product_id":"s45hepes-500","title":"HEPES buffer (1M)，pH7.3,Sterile, No phenol red Storage conditions: 2~8℃，500mL\/ Bottle, 10 Bottles\/Case","description":"\u003cstyle\u003e\n  \/* isQuote *\/\n  div.swatch.clearfix {\n    display:  unset ;\n  }\n\n  \/* Hide sku *\/\n  .product-sku, \n  #leadtimetext {\n    display: none !important;\n  }\n\n  \/* Square brackets *\/\n  span.l_bracket:before {\n    content: \"[\";\n  }\n  span.r_bracket:after {\n    content: \"]\";\n  }\n\n  \/* Hazard Symbols *\/\n  .ghs-symbols-row {\n    display: flex;\n    flex-wrap: wrap;\n    gap: 10px;\n  }\n\n  .hazard-symbol-container {\n    width: 50px;\n  }\n\n  .hazard-symbol {\n    object-fit: contain;\n    aspect-ratio: 1 \/ 1;\n    width: 100%;\n  }\n\n  \/* Section *\/\n  .section-header {\n    font-size: 1.5rem;\n    font-weight: bold;\n    color: #239ab7;\n    background-color: #f2f2f2;\n    padding: 10px;\n    border-radius: 4px;\n  }\n\n  .section-row {\n    display: grid;\n    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class=\"row-key\"\u003eProduct Name\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003eHEPES buffer (1M)，pH7.3,Sterile, No phenol red Storage conditions: 2~8℃，500mL\/ Bottle, 10 Bottles\/Case\u003c\/div\u003e\n        \u003c\/div\u003e\n        \n        \n        \n        \u003cdiv class=\"section-row\"\u003e\n            \u003cdiv class=\"row-key\"\u003eBrand Name\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003eBiologix\u003c\/div\u003e\n        \u003c\/div\u003e\n        \n\n        \n        \u003cdiv class=\"section-row\"\u003e\n            \u003cdiv class=\"row-key\"\u003eProduct Number\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003eHEPES-500\u003c\/div\u003e\n        \u003c\/div\u003e\n        \n\n        \n\n        \n\n        \n\n        \n\n        \u003cdiv class=\"section-row\"\u003e\n            \u003cdiv class=\"row-key\"\u003eCertificate of Analysis (COA)​\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003e\n                \u003cdiv class=\"coa-search-container\"\u003e\n                    \u003cinput id=\"coa-link-input\" type=\"text\" placeholder=\"Enter Lot No.\"\u003e\n                    \u003cbutton id=\"coa-search-button\"\u003e\n                        \u003cdiv class=\"coa-search-button-content\"\u003e\n                            \u003csvg class=\"search-spin\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewbox=\"0 0 200 200\"\u003e\u003cradialgradient id=\"a7\" cx=\".66\" fx=\".66\" cy=\".3125\" fy=\".3125\" gradienttransform=\"scale(1.5)\"\u003e\u003cstop offset=\"0\" stop-color=\"#FFFFFF\"\u003e\u003c\/stop\u003e\u003cstop offset=\".3\" stop-color=\"#FFFFFF\" stop-opacity=\".9\"\u003e\u003c\/stop\u003e\u003cstop offset=\".6\" stop-color=\"#FFFFFF\" stop-opacity=\".6\"\u003e\u003c\/stop\u003e\u003cstop offset=\".8\" stop-color=\"#FFFFFF\" stop-opacity=\".3\"\u003e\u003c\/stop\u003e\u003cstop offset=\"1\" stop-color=\"#FFFFFF\" stop-opacity=\"0\"\u003e\u003c\/stop\u003e\u003c\/radialgradient\u003e\u003ccircle transform-origin=\"center\" fill=\"none\" stroke=\"url(#a7)\" stroke-width=\"15\" stroke-linecap=\"round\" stroke-dasharray=\"200 1000\" stroke-dashoffset=\"0\" cx=\"100\" cy=\"100\" r=\"70\"\u003e\u003canimatetransform type=\"rotate\" attributename=\"transform\" calcmode=\"spline\" dur=\"2\" values=\"360;0\" keytimes=\"0;1\" keysplines=\"0 0 1 1\" repeatcount=\"indefinite\"\u003e\u003c\/animatetransform\u003e\u003c\/circle\u003e\u003ccircle transform-origin=\"center\" fill=\"none\" opacity=\".2\" stroke=\"#FFFFFF\" stroke-width=\"15\" stroke-linecap=\"round\" cx=\"100\" cy=\"100\" r=\"70\"\u003e\u003c\/circle\u003e\u003c\/svg\u003e\n                            \u003cspan\u003eSearch\u003c\/span\u003e\n                        \u003c\/div\u003e\n                    \u003c\/button\u003e\n                    \u003cspan class=\"coa-not-found\"\u003eCOA not found\u003c\/span\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n\n    \u003c\/div\u003e\n\n    \u003c!-- General Info --\u003e\n    \n\n    \u003c!-- Properties --\u003e\n    \n\n    \u003c!-- Safety Info --\u003e\n    \n\n    \u003c!-- Application  --\u003e\n    \n    \u003ch2 class=\"section-header\"\u003eApplication\u003c\/h2\u003e\n    \u003cdiv class=\"html-area\"\u003e\n        \u003cp\u003eHEPES is a commonly used zwitterionic buffer that can maintain a constant pH range for a long time. The effective buffer range is pH 6.8-8.2, and pKa (25°C) = 7.5. HEPES solution (1M, pH7.3) is commonly used in cell culture, and the general working concentration is 10-25mM. Compared with the low pKa bicarbonate buffer, HEPES has a pKa of 7.5 and has good buffering capacity in the pH range of 7.2-7.6, which is suitable for cell culture that requires precise pH conditions. Since the buffering capacity of HEPES is independent of CO2 concentration, HEPES can also provide good buffering capacity in open culture or when cells are out of the CO2 incubator for a long time (such as cell observation). HEPES is the preferred buffer for many cell culture systems because HEPES has no permeability to cell membranes, has little effect on various biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorption. HEPES is non-toxic to cells at a concentration of 10-25mM, but may be toxic to some cells and affect cell growth when the concentration exceeds 40mM. The HEPES solution (1M, pH7.3, for cell culture) produced by Biologix can be directly used for cell culture and other purposes after filtration and sterilization, and no filtration or other sterilization treatment is required before use.\u003c\/p\u003e\n\u003cdiv\u003e \u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e \u003cp\u003e1. Often used in fine cell culture, the general working concentration is 10-25 mM.\u003c\/p\u003e \u003cp\u003e2. It has good buffering capacity.\u003c\/p\u003e \u003cp\u003e3. HEPES is stable in chemistry and enzymology.\u003c\/p\u003e \u003cp\u003e4. Has very low visible light and UV light absorption.\u003c\/p\u003e \u003cp\u003e5. Sterile and almost endotoxin-free.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e6. Filtration sterilization can be used directly for cell culture. Store at 2-8℃.\u003c\/p\u003e\n\u003cdiv\u003e \u003cp\u003e\u003cstrong\u003eProduct Nature\u003c\/strong\u003e\u003c\/p\u003e \u003ctable\u003e  \u003ctbody\u003e   \u003ctr\u003e    \u003ctd\u003ePH\u003c\/td\u003e    \u003ctd\u003e7.3±0.1\u003c\/td\u003e   \u003c\/tr\u003e   \u003ctr\u003e    \u003ctd\u003ePhenol red indicator\u003c\/td\u003e    \u003ctd\u003eNo phenol red\u003c\/td\u003e   \u003c\/tr\u003e   \u003ctr\u003e    \u003ctd\u003eSterile\u003c\/td\u003e    \u003ctd\u003eSterile\u003c\/td\u003e   \u003c\/tr\u003e   \u003ctr\u003e    \u003ctd\u003eOsmotic pressure\u003c\/td\u003e    \u003ctd\u003e\/\u003c\/td\u003e   \u003c\/tr\u003e   \u003ctr\u003e    \u003ctd\u003eEndotoxin\u003c\/td\u003e    \u003ctd\u003e\/\u003c\/td\u003e   \u003c\/tr\u003e   \u003ctr\u003e    \u003ctd\u003eConcentration (M)\u003c\/td\u003e    \u003ctd\u003e1\u003c\/td\u003e   \u003c\/tr\u003e  \u003c\/tbody\u003e \u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cstrong\u003eOrdering Information\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003c\/div\u003e\n\u003cdiv\u003e \u003ctable\u003e  \u003ctbody\u003e   \u003ctr\u003e    \u003ctd\u003eCat #\u003c\/td\u003e    \u003ctd\u003eProduct Name\u003c\/td\u003e    \u003ctd\u003eFormula\u003c\/td\u003e    \u003ctd\u003ePackage\u003c\/td\u003e   \u003c\/tr\u003e   \u003ctr\u003e    \u003ctd\u003eHEPES-500\u003c\/td\u003e    \u003ctd\u003eHEPES Buffer (1M）\u003c\/td\u003e    \u003ctd\u003e1 M HEPES\u003c\/td\u003e    \u003ctd\u003e500mL\/ Bottle, 10 Bottles\/Case\u003c\/td\u003e   \u003c\/tr\u003e   \u003ctr\u003e    \u003ctd\u003eHEPES-500-1\u003c\/td\u003e    \u003ctd\u003eHEPES Buffer (1M）\u003c\/td\u003e    \u003ctd\u003e1 M HEPES\u003c\/td\u003e    \u003ctd\u003e500mL\/ Bottle\u003c\/td\u003e   \u003c\/tr\u003e  \u003c\/tbody\u003e \u003c\/table\u003e\n\u003c\/div\u003e\n    \u003c\/div\u003e\n    \n\n    \u003c!-- Faq --\u003e\n    \n\n    \u003c!-- References --\u003e\n    \n\n\n    \u003cscript\u003e\n        const coaSearchButton = document.getElementById('coa-search-button');\n        coaSearchButton.addEventListener('click', async function() {\n            const coaLinkInput = document.getElementById('coa-link-input');\n            const spinElement = document.querySelector('.search-spin');\n            const notFoundElement = document.querySelector('.coa-not-found');\n\n            const lot = coaLinkInput.value.trim().toUpperCase();\n            if (lot.length \u003e 0) {\n                spinElement.style.display = 'block';\n                notFoundElement.style.display = 'none';\n\n                const url = `https:\/\/web.jkchemical.com\/partial\/coaFileLotNumber\/${lot}`;\n                await fetch(url).then((res) =\u003e {\n                    if (res.status === 200) {\n                        window.open(url, '_blank');\n                    } else {\n                        notFoundElement.style.display = 'block';\n                    }\n                }).finally(() =\u003e {\n                    spinElement.style.display = 'none';\n                });\n            }\n        });\n    \u003c\/script\u003e\n\n\n\u003c\/div\u003e","brand":"Biologix","offers":[{"title":"10×500ml","offer_id":45394027413694,"sku":"S45HEPES-500_500_ML","price":204.3,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0506\/0005\/0878\/files\/c2562c84d1750a1ff3db472ee4f855ce.jpg?v=1776400786","url":"https:\/\/www.jk-sci.com\/products\/s45hepes-500","provider":"J\u0026K Scientific LLC","version":"1.0","type":"link"}