{"product_id":"j5300194","title":"Isopropyl-b-D-thiogalactopyranoside, dioxane free | 367-93-1","description":"\u003cstyle\u003e\n  \/* isQuote *\/\n  div.swatch.clearfix {\n    display:  unset ;\n  }\n\n  \/* Hide sku *\/\n  .product-sku, \n  #leadtimetext {\n    display: none !important;\n  }\n\n  \/* Square brackets *\/\n  span.l_bracket:before {\n    content: \"[\";\n  }\n  span.r_bracket:after {\n    content: \"]\";\n  }\n\n  \/* Hazard Symbols *\/\n  .ghs-symbols-row {\n    display: flex;\n    flex-wrap: wrap;\n    gap: 10px;\n  }\n\n  .hazard-symbol-container {\n    width: 50px;\n  }\n\n  .hazard-symbol {\n    object-fit: contain;\n    aspect-ratio: 1 \/ 1;\n    width: 100%;\n  }\n\n  \/* Section *\/\n  .section-header {\n    font-size: 1.5rem;\n    font-weight: bold;\n    color: #239ab7;\n    background-color: #f2f2f2;\n    padding: 10px;\n    border-radius: 4px;\n  }\n\n  .section-row {\n    display: grid;\n    grid-template-columns: repeat(8, 1fr);\n    padding: 6px 10px;\n   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class=\"row-value\"\u003eIsopropyl-b-D-thiogalactopyranoside, dioxane free\u003c\/div\u003e\n        \u003c\/div\u003e\n        \n        \n        \n        \u003cdiv class=\"section-row\"\u003e\n            \u003cdiv class=\"row-key\"\u003eBrand Name\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003eChem Impex\u003c\/div\u003e\n        \u003c\/div\u003e\n        \n\n        \n        \u003cdiv class=\"section-row\"\u003e\n            \u003cdiv class=\"row-key\"\u003eProduct Number\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003e00194\u003c\/div\u003e\n        \u003c\/div\u003e\n        \n\n        \n        \u003cdiv class=\"section-row\"\u003e\n            \u003cdiv class=\"row-key\"\u003eCAS\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003e367-93-1\u003c\/div\u003e\n        \u003c\/div\u003e\n        \n\n        \n\n        \n\n        \n\n        \u003cdiv class=\"section-row\"\u003e\n            \u003cdiv class=\"row-key\"\u003eCertificate of Analysis (COA)​\u003c\/div\u003e\n            \u003cdiv class=\"row-value\"\u003e\n                \u003cdiv class=\"coa-search-container\"\u003e\n                    \u003cinput id=\"coa-link-input\" type=\"text\" placeholder=\"Enter Lot No.\"\u003e\n                    \u003cbutton id=\"coa-search-button\"\u003e\n                        \u003cdiv class=\"coa-search-button-content\"\u003e\n                            \u003csvg class=\"search-spin\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" viewbox=\"0 0 200 200\"\u003e\u003cradialgradient id=\"a7\" cx=\".66\" fx=\".66\" cy=\".3125\" fy=\".3125\" gradienttransform=\"scale(1.5)\"\u003e\u003cstop offset=\"0\" stop-color=\"#FFFFFF\"\u003e\u003c\/stop\u003e\u003cstop offset=\".3\" stop-color=\"#FFFFFF\" stop-opacity=\".9\"\u003e\u003c\/stop\u003e\u003cstop offset=\".6\" stop-color=\"#FFFFFF\" stop-opacity=\".6\"\u003e\u003c\/stop\u003e\u003cstop offset=\".8\" stop-color=\"#FFFFFF\" stop-opacity=\".3\"\u003e\u003c\/stop\u003e\u003cstop offset=\"1\" stop-color=\"#FFFFFF\" stop-opacity=\"0\"\u003e\u003c\/stop\u003e\u003c\/radialgradient\u003e\u003ccircle transform-origin=\"center\" fill=\"none\" stroke=\"url(#a7)\" stroke-width=\"15\" stroke-linecap=\"round\" stroke-dasharray=\"200 1000\" stroke-dashoffset=\"0\" cx=\"100\" cy=\"100\" r=\"70\"\u003e\u003canimatetransform type=\"rotate\" attributename=\"transform\" calcmode=\"spline\" dur=\"2\" values=\"360;0\" keytimes=\"0;1\" keysplines=\"0 0 1 1\" repeatcount=\"indefinite\"\u003e\u003c\/animatetransform\u003e\u003c\/circle\u003e\u003ccircle transform-origin=\"center\" fill=\"none\" opacity=\".2\" stroke=\"#FFFFFF\" stroke-width=\"15\" stroke-linecap=\"round\" cx=\"100\" cy=\"100\" r=\"70\"\u003e\u003c\/circle\u003e\u003c\/svg\u003e\n                            \u003cspan\u003eSearch\u003c\/span\u003e\n                        \u003c\/div\u003e\n                    \u003c\/button\u003e\n                    \u003cspan class=\"coa-not-found\"\u003eCOA not found\u003c\/span\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n\n    \u003c\/div\u003e\n\n    \u003c!-- General Info --\u003e\n    \n\n    \u003c!-- Properties --\u003e\n    \n\n    \u003c!-- Safety Info --\u003e\n    \n\n    \u003c!-- Application  --\u003e\n    \n    \u003ch2 class=\"section-header\"\u003eApplication\u003c\/h2\u003e\n    \u003cdiv class=\"html-area\"\u003e\n        \u003ch2\u003eProduct Introduction\u003c\/h2\u003e\n\u003cp\u003eIsopropyl-β-D-thiogalactopyranoside (IPTG) is a widely used non-metabolizable inducer of the lac operon in molecular biology. It is a synthetic analog of lactose that can effectively induce gene expression controlled by lac promoter-based systems.\u003c\/p\u003e\n\u003cp\u003eUnlike lactose, IPTG is not hydrolyzed by β-galactosidase, allowing for stable and sustained induction of recombinant protein expression. It binds to the lac repressor (LacI), releasing it from the operator region and enabling transcription of downstream genes.\u003c\/p\u003e\n\u003cp\u003eDue to its reliability and efficiency, IPTG is extensively used in recombinant protein expression, gene regulation studies, and bacterial expression systems, especially in E. coli.\u003c\/p\u003e\n\u003ch2\u003eMechanism of Reaction\u003c\/h2\u003e\n\u003cp\u003eIsopropyl-β-D-thiogalactopyranoside functions as a lac operon inducer.In molecular systems:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eIPTG binds to the lac repressor (LacI)\u003c\/li\u003e\n\u003cli\u003eCauses conformational change in LacI\u003c\/li\u003e\n\u003cli\u003eReleases LacI from the lac operator\u003c\/li\u003e\n\u003cli\u003eInitiates transcription of downstream genes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis makes IPTG ideal for controlled gene expression systems.\u003c\/p\u003e\n\u003ch2\u003eKey Research Applications\u003c\/h2\u003e\n\u003ch3\u003e1.Recombinant Protein Expression\u003c\/h3\u003e\n\u003cp\u003eIPTG is widely used to induce protein expression in bacterial systems.Typical applications include:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eExpression of recombinant proteins in E. coli\u003c\/li\u003e\n\u003cli\u003eInduction of lac promoter-controlled genes\u003c\/li\u003e\n\u003cli\u003eOptimization of expression conditions\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e2.Gene Regulation Studies\u003c\/h3\u003e\n\u003cp\u003eIt is commonly used in studies of gene expression and regulation.Applications include:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003ePromoter activity analysis\u003c\/li\u003e\n\u003cli\u003eGene function studies\u003c\/li\u003e\n\u003cli\u003eRegulatory pathway research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e3.Molecular Cloning and Reporter Systems\u003c\/h3\u003e\n\u003cp\u003eIPTG plays a key role in molecular biology techniques.Applications include:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eBlue-white screening (with X-Gal)\u003c\/li\u003e\n\u003cli\u003eReporter gene assays\u003c\/li\u003e\n\u003cli\u003ePlasmid-based expression systems\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e4.Synthetic Biology and Biotechnology\u003c\/h3\u003e\n\u003cp\u003eWidely used in engineered biological systems.Applications include:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eSynthetic gene circuit design\u003c\/li\u003e\n\u003cli\u003eControlled expression systems\u003c\/li\u003e\n\u003cli\u003eIndustrial biotechnology processes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eRecommended Experimental Conditions\u003c\/h2\u003e\n\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eParameter\u003c\/td\u003e\n\u003ctd\u003eRecommended Conditions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWorking Concentration\u003c\/td\u003e\n\u003ctd\u003eTypically 0.1–1 mM (optimize as needed)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInduction Method\u003c\/td\u003e\n\u003ctd\u003eAddition to culture medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHost System\u003c\/td\u003e\n\u003ctd\u003eCommonly \u003ci\u003eE. coli\u003c\/i\u003e expression systems\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTemperature\u003c\/td\u003e\n\u003ctd\u003e16–37 °C (depending on expression strategy)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay Format\u003c\/td\u003e\n\u003ctd\u003eLiquid culture, agar plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003ci\u003eOptimal conditions depend on vector, host strain, and target protein.\u003c\/i\u003e\u003c\/p\u003e\n\u003ch2\u003eAdvantages in Enzyme Assays\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eStrong and stable induction capability\u003c\/li\u003e\n\u003cli\u003eNon-metabolizable (consistent expression levels)\u003c\/li\u003e\n\u003cli\u003eEasy to use and widely compatible\u003c\/li\u003e\n\u003cli\u003eSuitable for a wide range of expression systems\u003c\/li\u003e\n\u003cli\u003eHighly reproducible results\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eResearch Areas\u003c\/h2\u003e\n\u003cp\u003eResearchers working in the following fields may benefit from this compound:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMolecular biology and gene expression research\u003c\/li\u003e\n\u003cli\u003eRecombinant protein production\u003c\/li\u003e\n\u003cli\u003eSynthetic biology and genetic engineering\u003c\/li\u003e\n\u003cli\u003eMicrobiology and bacterial systems\u003c\/li\u003e\n\u003cli\u003eBiotechnology and industrial enzyme production\u003c\/li\u003e\n\u003c\/ul\u003e\n    \u003c\/div\u003e\n    \n\n    \u003c!-- Faq --\u003e\n    \n    \u003ch2 class=\"section-header\"\u003eFAQ\u003c\/h2\u003e\n    \u003cdiv class=\"html-area\"\u003e\n        \u003cp\u003e\u003cstrong\u003eWhat is IPTG used for?\u003c\/strong\u003e\u003cbr\u003eIt is used to induce gene expression in lac operon-based systems, especially in E. coli.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhy is IPTG preferred over lactose?\u003c\/strong\u003e\u003cbr\u003eBecause it is non-metabolizable, providing stable and continuous induction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat concentration of IPTG should be used?\u003c\/strong\u003e\u003cbr\u003eTypically 0.1–1 mM, but optimization is recommended depending on the system.\u003c\/p\u003e\n    \u003c\/div\u003e\n    \n\n    \u003c!-- References --\u003e\n    \n\n\n    \u003cscript\u003e\n        const coaSearchButton = document.getElementById('coa-search-button');\n        coaSearchButton.addEventListener('click', async function() {\n            const coaLinkInput = document.getElementById('coa-link-input');\n            const 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