Chemical cross-linking coupled with mass spectrometry (CXMS) has been booming in the past decade and is widely used in protein structure identification and protein interaction analysis. However, the chemical cross-linking agents available for CXMS technology are limited and the cross-linking efficiency is not high enough, which limits the application of this technology [1][1][1]. (B) Purification process of Leiker-binding peptides (C) Three ways of Leiker-binding peptides
Compared with traditional cross-linking agent BS3, Leiker has the following advantages:
It can effectively enrich more than 97% of peptides to achieve efficient analysis of complex protein samples.
Leiker allowed near 100% enrichment of target peptides from a cross-linked ten-protein mixture diluted with increasing amounts of non-cross-linked E. coli lysates.
Cross-linking efficiency is more than 10 times higher
CXMS analyses of E. coli and C. elegans lysates. The best protein-protein interaction cluster extracted from the Leiker-identified or BS3-identified (Yang et al., 2012) inter-links from E.coli whole-cell lysates.
Deuterated isotope-labeled d6-Leiker enables CXMS quantitative analysis Example: Quantitative analysis of L7Ae-RNA complexes
Reciprocal labeling of RNA-free (F) and RNA-bound (B) L7Ae with [d0]/[d6]-Leiker.
Bailingwei provides scientists around the world with the Leiker series of products transformed by Professor Lei Xiaoguang’s scientific research results:
5mg, 10mg
5mg, 10mg